RRC ID |
58933
|
著者 |
Abe F, Haque E, Hisano H, Tanaka T, Kamiya Y, Mikami M, Kawaura K, Endo M, Onishi K, Hayashi T, Sato K.
|
タイトル |
Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat.
|
ジャーナル |
Cell Rep
|
Abstract |
Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley.
|
巻・号 |
28(5)
|
ページ |
1362-1369.e4
|
公開日 |
2019-7-30
|
DOI |
10.1016/j.celrep.2019.06.090
|
PII |
S2211-1247(19)30870-8
|
PMID |
31365876
|
MeSH |
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Gene Editing*
Gene Knockout Techniques
Genes, Recessive*
Mutation*
Plant Dormancy / genetics*
Plant Proteins / genetics
Plant Proteins / metabolism
Seeds* / genetics
Seeds* / metabolism
Triticum* / genetics
Triticum* / metabolism
|
IF |
8.109
|
引用数 |
9
|
リソース情報 |
オオムギ |
BAC clone sequence
LC054174
LC054175 |