RRC ID 58979
Author Utami ST, Indriani CI, Bowolaksono A, Yaguchi T, Chen X, Niimi K, Niimi M, Kajiwara S.
Title Identification and functional characterization of Penicillium marneffei major facilitator superfamily (MFS) transporters.
Journal Biosci Biotechnol Biochem
Abstract PENICILLIUM MARNEFFEI:is a thermally dimorphic fungus that causes penicilliosis, and become the third-most-common opportunistic fungal infection in immunocompromised patients in Southeast Asia. Azoles and amphotericin B have been introduced for the treatment, however, it is important to investigate possible mechanisms of azole resistance for future treatment failure. We identified 177 putative MFS transporters and classified into 17 subfamilies. Among those, members of the Drug:H+ antiporter 1 subfamily are known to confer resistance to antifungals. Out of 39 paralogs, three (encoded by PmMDR1, PmMDR2, and PmMDR3) were heterologously overexpressed in S. cerevisiae AD∆ conferred resistance to various drugs and compounds including azoles, albeit to different degrees. PmMDR1-expressing strain showed resistance to the broadest range of drugs, followed by the PmMDR3, and PmMDR2 conferred weak resistance to a limited range of drugs. We conclude that PmMDR1 and PmMDR3, may be able to serve as multidrug efflux pumps.
Volume 84(7)
Pages 1373-1383
Published 2020-7-1
DOI 10.1080/09168451.2020.1732185
PMID 32163007
MeSH ATP Binding Cassette Transporter, Subfamily B / chemistry* ATP Binding Cassette Transporter, Subfamily B / genetics ATP Binding Cassette Transporter, Subfamily B / metabolism* Amino Acid Sequence Amphotericin B / pharmacology* Amphotericin B / therapeutic use Antifungal Agents / pharmacology* Asia, Southeastern / epidemiology Candida albicans / drug effects Candida albicans / metabolism Drug Resistance, Fungal / drug effects Humans Immunocompromised Host Microbial Sensitivity Tests Mycoses / drug therapy Mycoses / epidemiology Mycoses / metabolism* Mycoses / microbiology Phylogeny Saccharomyces cerevisiae / drug effects Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Talaromyces / drug effects Talaromyces / metabolism* Transcriptome Triazoles / pharmacology* Triazoles / therapeutic use
IF 1.516
Times Cited 0
Pathogenic eukaryotic microorganisms IFM 60996