RRC ID 5898
著者 Cai S, Dong X.
タイトル Cellulosilyticum ruminicola gen. nov., sp. nov., isolated from the rumen of yak, and reclassification of Clostridium lentocellum as Cellulosilyticum lentocellum comb. nov.
ジャーナル Int J Syst Evol Microbiol
Abstract An obligate anaerobic, Gram-staining-negative, mesophilic, cellulolytic bacterium, strain H1(T), was isolated from the rumen content of yak. Cells were straight to slightly curved rods, 0.8-1.0 x 3.0-4.0 microm in size, non-motile and encapsulated with mucous materials. Elliptical and terminal spores that swelled the cells were produced occasionally. The strain grew at 25-45 degrees C (optimum, 38 degrees C) and pH 6.0-7.8 (optimum, pH 6.7). Cellulose, cellobiose, xylan, xylose and maltose were used as carbon and energy sources, but not glucose. Products from cellulose and cellobiose fermentation were formic acid, acetic acid, carbon dioxide and trace amounts of ethanol, lactic acid and succinic acid. The genomic DNA G+C content was 33.7+/-1.2 mol%. The predominant fatty acids were C(16 : 0) (27.1 %), C(14 : 0) (9.2 %) and iso-C( 16 : 0) (6.4%). Based on the 16S rRNA gene sequence analysis, strain H1(T) was affiliated to the clostridial rRNA cluster XIVb and showed the highest 16S rRNA gene sequence similarity to Clostridium lentocellum DSM 5427(T) (96.0 %). These two strains formed a distinct lineage of the family 'Lachnospiraceae '. Based on data from this polyphasic taxonomic study, a new genus, Cellulosilyticum gen. nov., is proposed. Cellulosilyticum ruminicola sp. nov. is proposed for strain H1(T). The type strain of Cellulosilyticum ruminicola sp. nov. is strain H1(T) (=CGMCC 1.5065(T)=JCM 14822(T)). Clostridium lentocellum was reclassified in the new genus as Cellulosilyticum lentocellum comb. nov. (type strain RHM5(T)=ATCC 49066( T)=DSM 5427(T)=NCIMB 11756(T)).
巻・号 60(Pt 4)
ページ 845-849
公開日 2010-4-1
DOI 10.1099/ijs.0.014712-0
PMID 19661493
MeSH Animals Bacterial Typing Techniques Base Composition Cattle / microbiology* Cellulose / metabolism Clostridium / classification* Clostridium / genetics Clostridium / physiology DNA, Ribosomal / analysis DNA, Ribosomal / genetics Fatty Acids / analysis Genes, rRNA Genotype Gram-Negative Bacteria / classification* Gram-Negative Bacteria / genetics Gram-Negative Bacteria / isolation & purification* Gram-Negative Bacteria / physiology Molecular Sequence Data Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Rumen / microbiology* Sequence Analysis, DNA Species Specificity
IF 2.415
引用数 27
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 14822