RRC ID 58994
Author Haque MR, Higashiura A, Nakagawa A, Hirowatari A, Furuya S, Yamamoto K.
Title Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori.
Journal FEBS Open Bio
Abstract The enzyme 5,10-methylenetetrahydrofolate dehydrogenase (MTHFD) is essential for the production of certain amino acids (glycine, serine, and methionine) and nucleic acids (thymidylate and purine). Here, we identified a cDNA encoding this enzyme from the silkworm Bombyx mori. The recombinant B. mori MTHFD (bmMTHFD) expressed in Escherichia coli recognized 5,10-methylenetetrahydrofolate and 5,10-methenyltetrahydrofolate as substrate in the presence of NADP + as well as NAD +. The bmMTHFD structure was determined at a resolution of 1.75 Å by X-ray crystallography. Site-directed mutagenesis indicated that the amino acid residue Tyr49 contributed to its catalytic activity. Our findings provide insight into the mechanism underlying the activity of MTHFD from B. mori and potentially other insects and may therefore facilitate the development of inhibitors specific to MTHFD as insecticides.
Volume 9(4)
Pages 618-628
Published 2019-4
DOI 10.1002/2211-5463.12595
PII FEB412595
PMID 30984537
PMC PMC6443876
MeSH Amino Acid Sequence Animals Bombyx / enzymology Bombyx / genetics* Bombyx / metabolism DNA, Complementary / chemistry DNA, Complementary / genetics DNA, Complementary / metabolism Escherichia coli / genetics Insect Proteins / chemistry Insect Proteins / genetics* Insect Proteins / metabolism Methylenetetrahydrofolate Dehydrogenase (NADP) / chemistry Methylenetetrahydrofolate Dehydrogenase (NADP) / genetics* Methylenetetrahydrofolate Dehydrogenase (NADP) / metabolism Molecular Structure Mutagenesis, Site-Directed Phylogeny Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Alignment
IF 1.959
Times Cited 3
Resource
Silkworms p50T