RRC ID 5901
著者 Castañeda-Carrión IN, Sheik CS, Krumholz LR.
タイトル Desulfovibrio africanus subsp. uniflagellum subsp. nov., a sulfate-reducing bacterium from a uranium-contaminated subsurface aquifer.
ジャーナル Int J Syst Evol Microbiol
Abstract The bacterial strain SR-1(T) was isolated from subsurface sediments of a uranium-contaminated site in Shiprock, New Mexico, USA. Cells are vibrioid and motile by means of a single polar flagellum. Strain SR-1(T) grows on sulfate, oxidizing formate, lactate and H2, but not malate, and ferments pyruvate. The DNA sequences of the 16S rRNA gene and the 16S-23S internal transcribed spacer of strain SR-1(T) showed 99.9 and 99.4 % similarity, respectively, to those of the type strain Desulfovibrio africanus DSM 2603(T). The DNA sequence of the ITS region is 300 bases in length and contains two tRNA genes (tRNA(Ile), tRNA(Ala)). The partial DNA sequence of the dsrAB gene showed 94.6 % amino acid sequence similarity to that of D. africanus. The DNA G+C content of strain SR-1(T) was 62.4 mol% and it showed 72 % DNA-DNA similarity to D. africanus. DNA typing methods that target gene clusters and whole genomes revealed characteristic genomic fingerprints for strain SR-1(T). A small plasmid was detected by gel electrophoresis. On the basis of distinct phenotypic and genotypic characteristics, strain SR-1(T) represents a novel subspecies of D. africanus, for which the name Desulfovibrio africanus subsp. uniflagellum subsp. nov. is proposed. The type strain is SR-1(T) (=JCM 15510(T) =LS KCTC 5649(T)).
巻・号 60(Pt 4)
ページ 880-886
公開日 2010-4-1
DOI 10.1099/ijs.0.006668-0
PMID 19661495
MeSH Bacterial Typing Techniques Base Composition DNA, Ribosomal Spacer / analysis DNA, Ribosomal Spacer / genetics Desulfovibrio africanus / classification* Desulfovibrio africanus / genetics Desulfovibrio africanus / isolation & purification Desulfovibrio africanus / metabolism Fresh Water / chemistry Fresh Water / microbiology* Genes, rRNA Genotype Geologic Sediments / chemistry Geologic Sediments / microbiology* Molecular Sequence Data New Mexico Phenotype Phylogeny RNA, Ribosomal, 16S / genetics RNA, Ribosomal, 23S / genetics Sequence Analysis, DNA Soil Pollutants / metabolism Species Specificity Sulfates / metabolism* Sulfur-Reducing Bacteria / classification Sulfur-Reducing Bacteria / genetics Sulfur-Reducing Bacteria / isolation & purification Sulfur-Reducing Bacteria / metabolism Uranium* / metabolism
IF 2.415
引用数 2
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 15510