論文 - 詳細
| RRC ID | 5903 |
|---|---|
| 著者 | Echigo A, Minegishi H, Mizuki T, Kamekura M, Usami R. |
| タイトル | Geomicrobium halophilum gen. nov., sp. nov., a moderately halophilic and alkaliphilic bacterium isolated from soil. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
Two moderately halophilic and alkaliphilic bacteria, designated strains BH1(T) and HN5, were isolated from forest soil and garden soil, respectively, in Japan. Cells of strains BH1(T) and HN5 were non-motile, aerobic, bean-shaped, formed irregular clusters with 2-20 cells, Gram-positive and contained A1gamma, meso-diaminopimelic acid-type murein. Spore formation was not detected. Growth occurred in 5-25 % (w/v) NaCl (optimum, 10-15 %, w/v), at pH 6.0-10.0 (optimum, pH 8.5-9.0) and at 20-40 degrees C (optimum, 30 degrees C). The predominant isoprenoid quinones were menaquinone-7 and menaquinone-6. The phospholipids were diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids were i-C(15 : 0), i-C(17 : 0) and i-C(18 : 0). The DNA G+C content of strains BH1(T) and HN5 was 45 and 46 mol%, respectively. The 16S rRNA gene sequences of the two strains were 99.9 % similar. DNA-DNA hybridization results indicated high levels of relatedness (88 and 85 % reciprocally). Similarities with recognized species were less than 90.2 %. The phylogenetic and phenotypic characteristics indicated that strains BH1(T) and HN5 represent a novel species in a new genus, for which the name Geomicrobium halophilum gen. nov., sp. nov. is proposed. The type strain is BH1(T) (=JCM 15647(T)=DSM 21769(T)). |
| 巻・号 | 60(Pt 4) |
| ページ | 990-995 |
| 公開日 | 2010-4-1 |
| DOI | 10.1099/ijs.0.013268-0 |
| PMID | 19666816 |
| MeSH | Aerobiosis Bacterial Typing Techniques Base Composition DNA, Ribosomal / analysis DNA, Ribosomal / genetics Fatty Acids / analysis Genes, rRNA Genotype Gram-Positive Bacteria / classification* Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Hydrogen-Ion Concentration Japan Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride* Soil Microbiology* Species Specificity |
| IF | 2.415 |
| 引用数 | 13 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 15647 |