RRC ID 59060
著者 Jindal S, Thampy H, Day PJR, Kell DB.
タイトル Very rapid flow cytometric assessment of antimicrobial susceptibility during the apparent lag phase of microbial (re)growth.
ジャーナル Microbiology (Reading)
Abstract Rapid changes in the number and flow cytometric behaviour of cells of E. coli taken from a stationary phase and inoculated into rich medium.Cells of E. coli were grown in LB medium, taken from a stationary phase of 2-4 h, and re-inoculated into fresh media at a concentration (105 ml-1 or lower) characteristic of bacteriuria. Flow cytometry was used to assess how quickly we could detect changes in cell size, number, membrane energization (using a carbocyanine dye) and DNA distribution. It transpired that while the lag phase observable macroscopically via bulk OD measurements could be as long as 4 h, the true lag phase could be less than 15-20 min, and was accompanied by many observable biochemical changes. Antibiotics to which the cells were sensitive affected these changes within 20 min of re-inoculation, providing the possibility of a very rapid antibiotic susceptibility test on a timescale compatible with a visit to a GP clinic. The strategy was applied successfully to genuine potential urinary tract infection (UTI) samples taken from a doctor's surgery. The methods developed could prove of considerable value in ensuring the correct prescription and thereby lowering the spread of antimicrobial resistance.
巻・号 165(4)
ページ 439-454
公開日 2019-4-1
DOI 10.1099/mic.0.000777
PMID 30741628
MeSH Anti-Bacterial Agents / pharmacology* Anti-Bacterial Agents / therapeutic use Bacteria / drug effects* Bacteria / growth & development* Bacteria / isolation & purification Bacteriuria / drug therapy Bacteriuria / microbiology Culture Media Flow Cytometry* Humans Microbial Sensitivity Tests / methods* Urinary Tract Infections / drug therapy Urinary Tract Infections / microbiology*
IF 1.922
引用数 1
リソース情報
原核生物(大腸菌) ASKA (-) collection