RRC ID 59115
Author Kong J, Han H, Bergalet J, Bouvrette LPB, Hernández G, Moon NS, Vali H, Lécuyer É, Lasko P.
Title A ribosomal protein S5 isoform is essential for oogenesis and interacts with distinct RNAs in Drosophila melanogaster.
Journal Sci Rep
Abstract In Drosophila melanogaster there are two genes encoding ribosomal protein S5, RpS5a and RpS5b. Here, we demonstrate that RpS5b is required for oogenesis. Females lacking RpS5b produce ovaries with numerous developmental defects that undergo widespread apoptosis in mid-oogenesis. Females lacking germline RpS5a are fully fertile, but germline expression of interfering RNA targeting germline RpS5a in an RpS5b mutant background worsened the RpS5b phenotype and blocked oogenesis before egg chambers form. A broad spectrum of mRNAs co-purified in immunoprecipitations with RpS5a, while RpS5b-associated mRNAs were specifically enriched for GO terms related to mitochondrial electron transport and cellular metabolic processes. Consistent with this, RpS5b mitochondrial fractions are depleted for proteins linked to oxidative phosphorylation and mitochondrial respiration, and RpS5b mitochondria tended to form large clusters and had more heterogeneous morphology than those from controls. We conclude that RpS5b-containing ribosomes preferentially associate with particular mRNAs and serve an essential function in oogenesis.
Volume 9(1)
Pages 13779
Published 2019-9-24
DOI 10.1038/s41598-019-50357-z
PII 10.1038/s41598-019-50357-z
PMID 31551467
PMC PMC6760144
MeSH Animals Animals, Genetically Modified / genetics Apoptosis / genetics Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Female Germ Cells / physiology Mitochondria / genetics Oocytes / physiology Oogenesis / genetics* Ovary / physiology Protein Isoforms / genetics* RNA / genetics* Ribosomal Proteins / genetics* Ribosomes / genetics*
IF 3.998
Times Cited 1
Drosophila DGRC#101079 DGRC#109551