| RRC ID |
59117
|
| 著者 |
Sato K, Ahsan MT, Ote M, Koganezawa M, Yamamoto D.
|
| タイトル |
Calmodulin-binding transcription factor shapes the male courtship song in Drosophila.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Males of the Drosophila melanogaster mutant croaker (cro) generate a polycyclic pulse song dissimilar to the monocyclic songs typical of wild-type males during courtship. However, cro has not been molecularly mapped to any gene in the genome. We demonstrate that cro is a mutation in the gene encoding the Calmodulin-binding transcription factor (Camta) by genetic complementation tests with chromosomal deficiencies, molecular cloning of genomic fragments that flank the cro-mutagenic P-insertion, and phenotypic rescue of the cro mutant phenotype by Camta+-encoding cDNA as well as a BAC clone containing the gene for Camta. We further show that knockdown of the Camta-encoding gene phenocopies cro mutant songs when targeted to a subset of fruitless-positive neurons that include the mcALa and AL1 clusters in the brain. cro-GAL4 and an anti-Camta antibody labeled a large number of brain neurons including mcALa. We conclude that the Camta-encoding gene represents the cro locus, which has been implicated in a species-specific difference in courtship songs between D. sechellia and simulans.
|
| 巻・号 |
15(7)
|
| ページ |
e1008309
|
| 公開日 |
2019-7-1
|
| DOI |
10.1371/journal.pgen.1008309
|
| PII |
PGENETICS-D-19-00863
|
| PMID |
31344027
|
| PMC |
PMC6690551
|
| MeSH |
Animals
Brain / metabolism
Calmodulin / metabolism*
Courtship
Drosophila Proteins / genetics*
Drosophila Proteins / metabolism
Drosophila melanogaster / physiology*
Genetic Complementation Test
Male
Sexual Behavior, Animal / physiology*
Species Specificity
Trans-Activators / genetics*
Trans-Activators / metabolism
Vocalization, Animal
|
| IF |
5.175
|
| 引用数 |
1
|
| リソース情報 |
| ショウジョウバエ |
DGRC#150124
DGRC#104266 |
