RRC ID 59117
Author Sato K, Ahsan MT, Ote M, Koganezawa M, Yamamoto D.
Title Calmodulin-binding transcription factor shapes the male courtship song in Drosophila.
Journal PLoS Genet
Abstract Males of the Drosophila melanogaster mutant croaker (cro) generate a polycyclic pulse song dissimilar to the monocyclic songs typical of wild-type males during courtship. However, cro has not been molecularly mapped to any gene in the genome. We demonstrate that cro is a mutation in the gene encoding the Calmodulin-binding transcription factor (Camta) by genetic complementation tests with chromosomal deficiencies, molecular cloning of genomic fragments that flank the cro-mutagenic P-insertion, and phenotypic rescue of the cro mutant phenotype by Camta+-encoding cDNA as well as a BAC clone containing the gene for Camta. We further show that knockdown of the Camta-encoding gene phenocopies cro mutant songs when targeted to a subset of fruitless-positive neurons that include the mcALa and AL1 clusters in the brain. cro-GAL4 and an anti-Camta antibody labeled a large number of brain neurons including mcALa. We conclude that the Camta-encoding gene represents the cro locus, which has been implicated in a species-specific difference in courtship songs between D. sechellia and simulans.
Volume 15(7)
Pages e1008309
Published 2019-7-1
DOI 10.1371/journal.pgen.1008309
PII PGENETICS-D-19-00863
PMID 31344027
PMC PMC6690551
MeSH Animals Brain / metabolism Calmodulin / metabolism* Courtship Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / physiology* Genetic Complementation Test Male Sexual Behavior, Animal / physiology* Species Specificity Trans-Activators / genetics* Trans-Activators / metabolism Vocalization, Animal
IF 5.224
Times Cited 1
Resource
Drosophila DGRC#150124 DGRC#104266