論文 - 詳細
| RRC ID | 59196 |
|---|---|
| 著者 | Nolivos S, Cayron J, Dedieu A, Page A, Delolme F, Lesterlin C. |
| タイトル | Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer. |
| ジャーナル | Science |
| Abstract |
Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor-conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action. |
| 巻・号 | 364(6442) |
| ページ | 778-782 |
| 公開日 | 2019-5-24 |
| DOI | 10.1126/science.aav6390 |
| PII | 364/6442/778 |
| PMID | 31123134 |
| MeSH | Anti-Bacterial Agents / pharmacology Antiporters / antagonists & inhibitors Antiporters / biosynthesis Antiporters / genetics Bacterial Proteins / antagonists & inhibitors Bacterial Proteins / biosynthesis Bacterial Proteins / genetics Carrier Proteins / genetics Carrier Proteins / physiology* Conjugation, Genetic DNA, Single-Stranded Drug Resistance, Multiple, Bacterial / genetics* Escherichia coli / drug effects Escherichia coli / genetics Escherichia coli / physiology* Escherichia coli Proteins / genetics Escherichia coli Proteins / physiology* F Factor / genetics F Factor / physiology* Microscopy Protein Biosynthesis / drug effects Tetracycline / pharmacology |
| IF | 41.063 |
| 引用数 | 15 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9806 |