RRC ID 59272
Author Karpf S, Riche CT, Di Carlo D, Goel A, Zeiger WA, Suresh A, Portera-Cailliau C, Jalali B.
Title Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates.
Journal Nat Commun
Abstract Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging speeds, however, mechanical scanners still limit the acquisition rates to typically 10-100 frames per second. Here we present a high-speed non-linear microscope achieving kilohertz frame rates by employing pulse-modulated, rapidly wavelength-swept lasers and inertia-free beam steering through angular dispersion. In combination with a high bandwidth, single-photon sensitive detector, this enables recording of fluorescent lifetimes at speeds of 88 million pixels per second. We show high resolution, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging flow cytometry with a digitally reconfigurable laser, imaging system and data acquisition system. These high speeds should enable high-speed and high-throughput image-assisted cell sorting.
Volume 11(1)
Pages 2062
Published 2020-4-28
DOI 10.1038/s41467-020-15618-w
PII 10.1038/s41467-020-15618-w
PMID 32346060
PMC PMC7188897
MeSH Euglena / cytology Imaging, Three-Dimensional Microscopy, Fluorescence, Multiphoton* Time Factors
IF 11.878
Times Cited 1
Algae NIES-48