| RRC ID |
59287
|
| 著者 |
Wu K, Zheng Y, Wu Q, Chen H, Fu S, Kan B, Long Y, Ni X, Tu J.
|
| タイトル |
Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology.
|
| ジャーナル |
J Microbiol
|
| Abstract |
In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. Parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography methods and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical 'fried-egg' shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a 'pancake' shape (no significant difference between the centre and the edge) of the cqsA deleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.
|
| 巻・号 |
57(12)
|
| ページ |
1105-1114
|
| 公開日 |
2019-12-1
|
| DOI |
10.1007/s12275-019-9379-x
|
| PII |
10.1007/s12275-019-9379-x
|
| PMID |
31686391
|
| MeSH |
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Cloning, Molecular
DNA, Bacterial / genetics
Escherichia coli / genetics
Escherichia coli / metabolism
Gene Deletion
Gene Expression Regulation, Bacterial
Genes, Bacterial / genetics
Phenotype
Quorum Sensing*
Signal Transduction*
Transcription Factors / metabolism
Vibrio / genetics
Vibrio / growth & development
Vibrio / metabolism
Vibrio parahaemolyticus / genetics
Vibrio parahaemolyticus / growth & development
Vibrio parahaemolyticus / metabolism*
|
| IF |
2.319
|
| 引用数 |
1
|
| リソース情報 |
| 病原細菌 |
Vibrio parahaemolyticus RIMD2210633 |
