RRC ID 59287
Author Wu K, Zheng Y, Wu Q, Chen H, Fu S, Kan B, Long Y, Ni X, Tu J.
Title Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology.
Journal J. Microbiol.
Abstract In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. Parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography methods and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical 'fried-egg' shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a 'pancake' shape (no significant difference between the centre and the edge) of the cqsA deleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.
Volume 57(12)
Pages 1105-1114
Published 2019-12
DOI 10.1007/s12275-019-9379-x
PII 10.1007/s12275-019-9379-x
PMID 31686391
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cloning, Molecular DNA, Bacterial / genetics Escherichia coli / genetics Escherichia coli / metabolism Gene Deletion Gene Expression Regulation, Bacterial Genes, Bacterial / genetics Phenotype Quorum Sensing* Signal Transduction* Transcription Factors / metabolism Vibrio / genetics Vibrio / growth & development Vibrio / metabolism Vibrio parahaemolyticus / genetics Vibrio parahaemolyticus / growth & development Vibrio parahaemolyticus / metabolism*
IF 2.319
Resource
Pathogenic bacteria Vibrio parahaemolyticus RIMD2210633