| RRC ID |
59318
|
| 著者 |
Billmyre KK, Doebley AL, Spichal M, Heestand B, Belicard T, Sato-Carlton A, Flibotte S, Simon M, Gnazzo M, Skop A, Moerman D, Carlton PM, Sarkies P, Ahmed S.
|
| タイトル |
The meiotic phosphatase GSP-2/PP1 promotes germline immortality and small RNA-mediated genome silencing.
|
| ジャーナル |
PLoS Genet
|
| Abstract |
Germ cell immortality, or transgenerational maintenance of the germ line, could be promoted by mechanisms that could occur in either mitotic or meiotic germ cells. Here we report for the first time that the GSP-2 PP1/Glc7 phosphatase promotes germ cell immortality. Small RNA-induced genome silencing is known to promote germ cell immortality, and we identified a separation-of-function allele of C. elegans gsp-2 that is compromised for germ cell immortality and is also defective for small RNA-induced genome silencing and meiotic but not mitotic chromosome segregation. Previous work has shown that GSP-2 is recruited to meiotic chromosomes by LAB-1, which also promoted germ cell immortality. At the generation of sterility, gsp-2 and lab-1 mutant adults displayed germline degeneration, univalents, histone methylation and histone phosphorylation defects in oocytes, phenotypes that mirror those observed in sterile small RNA-mediated genome silencing mutants. Our data suggest that a meiosis-specific function of GSP-2 ties small RNA-mediated silencing of the epigenome to germ cell immortality. We also show that transgenerational epigenomic silencing at hemizygous genetic elements requires the GSP-2 phosphatase, suggesting a functional link to small RNAs. Given that LAB-1 localizes to the interface between homologous chromosomes during pachytene, we hypothesize that small localized discontinuities at this interface could promote genomic silencing in a manner that depends on small RNAs and the GSP-2 phosphatase.
|
| 巻・号 |
15(3)
|
| ページ |
e1008004
|
| 公開日 |
2019-3-1
|
| DOI |
10.1371/journal.pgen.1008004
|
| PII |
PGENETICS-D-18-00406
|
| PMID |
30921322
|
| PMC |
PMC6456222
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Chromosomal Proteins, Non-Histone / metabolism
Chromosome Segregation
Genome
Germ Cells / metabolism*
Germ Cells / physiology
Meiosis / physiology
Meiotic Prophase I / physiology
Methylation
Phosphoric Monoester Hydrolases
Protein Phosphatase 1 / metabolism
Protein Phosphatase 1 / physiology*
RNA Interference / physiology
RNA, Small Interfering
|
| IF |
5.224
|
| 引用数 |
1
|
| リソース情報 |
| 線虫 |
tm301
tm1791
tm1200
tm1231
tm1857 |
