論文 - 詳細
| RRC ID | 59327 |
|---|---|
| 著者 | Chew YL, Grundy LJ, Brown AEX, Beets I, Schafer WR. |
| タイトル | Neuropeptides encoded by nlp-49 modulate locomotion, arousal and egg-laying behaviours in Caenorhabditis elegans via the receptor SEB-3. |
| ジャーナル | Philos Trans R Soc Lond B Biol Sci |
| Abstract |
Neuropeptide signalling has been implicated in a wide variety of biological processes in diverse organisms, from invertebrates to humans. The Caenorhabditis elegans genome has at least 154 neuropeptide precursor genes, encoding over 300 bioactive peptides. These neuromodulators are thought to largely signal beyond 'wired' chemical/electrical synapse connections, therefore creating a 'wireless' network for neuronal communication. Here, we investigated how behavioural states are affected by neuropeptide signalling through the G protein-coupled receptor SEB-3, which belongs to a bilaterian family of orphan secretin receptors. Using reverse pharmacology, we identified the neuropeptide NLP-49 as a ligand of this evolutionarily conserved neuropeptide receptor. Our findings demonstrate novel roles for NLP-49 and SEB-3 in locomotion, arousal and egg-laying. Specifically, high-content analysis of locomotor behaviour indicates that seb-3 and nlp-49 deletion mutants cause remarkably similar abnormalities in movement dynamics, which are reversed by overexpression of wild-type transgenes. Overexpression of NLP-49 in AVK interneurons leads to heightened locomotor arousal, an effect that is dependent on seb-3. Finally, seb-3 and nlp-49 mutants also show constitutive egg-laying in liquid medium and alter the temporal pattern of egg-laying in similar ways. Together, these results provide in vivo evidence that NLP-49 peptides act through SEB-3 to modulate behaviour, and highlight the importance of neuropeptide signalling in the control of behavioural states.This article is part of a discussion meeting issue 'Connectome to behaviour: modelling C. elegans at cellular resolution'. |
| 巻・号 | 373(1758) |
| 公開日 | 2018-9-10 |
| DOI | 10.1098/rstb.2017.0368 |
| PII | rstb.2017.0368 |
| PMID | 30201834 |
| PMC | PMC6158228 |
| MeSH | Animals Arousal* Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Locomotion Neuropeptides / genetics* Neuropeptides / metabolism Oviposition* Receptors, G-Protein-Coupled / genetics* Receptors, G-Protein-Coupled / metabolism |
| IF | 6.139 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1848 |