RRC ID 59328
Author Chisnell P, Parenteau TR, Tank E, Ashrafi K, Kenyon C.
Title The mTOR Target S6 Kinase Arrests Development in Caenorhabditis elegans When the Heat-Shock Transcription Factor Is Impaired.
Journal Genetics
Abstract The widely conserved heat-shock response, regulated by heat-shock transcription factors, is not only essential for cellular stress resistance and adult longevity, but also for proper development. However, the genetic mechanisms by which heat-shock transcription factors regulate development are not well understood. In Caenorhabditis elegans, we conducted an unbiased genetic screen to identify mutations that could ameliorate the developmental-arrest phenotype of a heat-shock factor mutant. Here, we show that loss of the conserved translational activator rsks-1/S6 kinase, a downstream effector of mechanistic Target of Rapamycin (mTOR) kinase, can rescue the developmental-arrest phenotype of hsf-1 partial loss-of-function mutants. Unexpectedly, we show that the rescue is not likely caused by reduced translation, nor by activation of any of a variety of stress-protective genes and pathways. Our findings identify an as-yet unexplained regulatory relationship between the heat-shock transcription factor and the mTOR pathway during C. elegans development.
Volume 210(3)
Pages 999-1009
Published 2018-11-1
DOI 10.1534/genetics.118.301533
PII genetics.118.301533
PMID 30228197
PMC PMC6218238
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Gene Deletion* Ribosomal Protein S6 Kinases, 70-kDa / deficiency Ribosomal Protein S6 Kinases, 70-kDa / genetics Ribosomal Protein S6 Kinases, 70-kDa / metabolism* TOR Serine-Threonine Kinases / metabolism* Transcription Factors / genetics* Transcription Factors / metabolism*
IF 3.564
Times Cited 1
C.elegans tm5213 tm5397