RRC ID 59329
Author Choi S, Ambros V.
Title The C. elegans heterochronic gene lin-28 coordinates the timing of hypodermal and somatic gonadal programs for hermaphrodite reproductive system morphogenesis.
Journal Development
Abstract C. elegans heterochronic genes determine the timing of expression of specific cell fates in particular stages of developing larvae. However, their broader roles in coordinating developmental events across diverse tissues have been less well investigated. Here, we show that loss of lin-28, a central heterochronic regulator of hypodermal development, causes reduced fertility associated with abnormal somatic gonadal morphology. In particular, the abnormal spermatheca-uterine valve morphology of lin-28(lf) hermaphrodites traps embryos in the spermatheca, which disrupts ovulation and causes embryonic lethality. The same genes that act downstream of lin-28 in the regulation of hypodermal developmental timing also act downstream of lin-28 in somatic gonadal morphogenesis and fertility. Importantly, we find that hypodermal expression, but not somatic gonadal expression, of lin-28 is sufficient for restoring normal somatic gonadal morphology in lin-28(lf) mutants. We propose that the abnormal somatic gonadal morphogenesis of lin-28(lf) hermaphrodites results from temporal discoordination between the accelerated hypodermal development and normally timed somatic gonadal development. Thus, our findings exemplify how a cell-intrinsic developmental timing program can also control proper development of other interacting tissues, presumably by cell non-autonomous signal(s). This article has an associated 'The people behind the papers' interview.
Volume 146(5)
Published 2019-3-7
DOI 10.1242/dev.164293
PII dev.164293
PMID 30745431
PMC PMC6432661
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / physiology* Cell Differentiation Cell Movement Female Fertilization Gene Expression Regulation, Developmental Gonads / physiology* Green Fluorescent Proteins / physiology Male Morphogenesis* Mutation Ovulation Plasmids RNA Interference RNA-Binding Proteins / physiology Repressor Proteins / physiology*
IF 5.763
Times Cited 2
C.elegans tm1071 tm545