RRC ID 59338
著者 Dillon J, Holden-Dye L, O'Connor V.
タイトル Yeast two-hybrid screening identifies MPZ-1 and PTP-1 as candidate scaffolding proteins of metabotropic glutamate receptors in Caenorhabditis elegans.
ジャーナル Invert Neurosci
Abstract The metabotropic glutamate receptors (mGluRs) are a class of G-protein-coupled receptor that undergo extensive interactions with scaffolding proteins, and this is intrinsic to their function as an important group of neuromodulators at glutamatergic synapses. The Caenorhabditis elegans nervous system expresses three metabotropic glutamate receptors, MGL-1, MGL-2 and MGL-3. Relatively little is known about how the function and signalling of these receptors is organised in C. elegans. To identify proteins that scaffold the MGL-1 receptor, we have conducted a yeast two-hybrid screen. Three of the interacting proteins, MPZ-1, NRFL-1 and PTP-1, displayed motifs characteristic of mammalian mGluR scaffolding proteins. Using cellular co-expression criterion, we show mpz-1 and ptp-1 exhibited overlapping expression patterns with subsets of mgl-1 neurons. This included neurones in the pharyngeal nervous system that control the feeding organ of the worm. The mGluR agonist L-CCG-I inhibits the activity of this network in wild-type worms, in an MGL-1 and dose-dependent manner. We utilised L-CCG-I to identify if MGL-1 function was disrupted in mutants with deletions in the mpz-1 gene. The mpz-1 mutants displayed a largely wild-type response to L-CCG-I, suggesting MGL-1 signalling is not overtly disrupted consistent with a non-obligatory modulatory function in receptor scaffolding. The selectivity of the protein interactions and overlapping expression identified here warrant further investigation of the functional significance of scaffolding of metabotropic glutamate receptor function.
巻・号 18(4)
ページ 16
公開日 2018-11-11
DOI 10.1007/s10158-018-0218-2
PII 10.1007/s10158-018-0218-2
PMID 30417267
MeSH Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry* Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / chemistry* Carrier Proteins / metabolism* Neurons / chemistry Neurons / metabolism Protein Tyrosine Phosphatases / chemistry* Protein Tyrosine Phosphatases / metabolism* Receptors, Metabotropic Glutamate / chemistry* Receptors, Metabotropic Glutamate / metabolism* Two-Hybrid System Techniques
IF 0.8
引用数 0
リソース情報
線虫 tm1136 tm1811