論文 - 詳細
| RRC ID | 59372 |
|---|---|
| 著者 | Li Y, Maine EM. |
| タイトル | The balance of poly(U) polymerase activity ensures germline identity, survival and development in Caenorhabditis elegans. |
| ジャーナル | Development |
| Abstract |
Poly(U) polymerases (PUPs) catalyze 3' uridylation of mRNAs and small RNAs, a modification often correlating with decreased RNA stability. We have investigated the importance of three proteins with in vitro PUP activity, PUP-1/CDE-1, PUP-2 and PUP-3, in C. elegans germline development. Genetic analysis indicates that PUP-1/CDE-1 and PUP-2 are developmentally redundant under conditions of temperature stress during which they ensure germline viability and development. Multiple lines of evidence indicate that pup-1/-2 double mutant germ cells fail to maintain their identity as distinct from soma. Consistent with phenotypic data, PUP-1 and PUP-2 are expressed in embryonic germ cell precursors and throughout germline development. The developmental importance of PUP activity is presumably in regulating gene expression as both a direct and indirect consequence of modifying target RNAs. PUP-3 is significantly overexpressed in the pup-1/-2 germline, and loss of pup-3 function partially suppresses pup-1/-2 germline defects. We conclude that one major function of PUP-1/-2 is to limit PUP-3 expression. Overall, the balance of PUP-1, PUP-2 and PUP-3 activities appears to ensure proper germline development. |
| 巻・号 | 145(19) |
| 公開日 | 2018-10-10 |
| DOI | 10.1242/dev.165944 |
| PII | 145/19/dev165944 |
| PMID | 30305273 |
| PMC | PMC6198469 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* DNA-Directed RNA Polymerases / genetics DNA-Directed RNA Polymerases / metabolism* Embryo, Nonmammalian / metabolism Embryonic Development* Female Fertilization Gene Expression Regulation, Developmental Genes, Reporter Germ Cells / metabolism* Green Fluorescent Proteins / metabolism Male Models, Biological Mutation / genetics Neurons / metabolism Spermatozoa / metabolism Survival Analysis Up-Regulation / genetics |
| IF | 5.763 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1021 tm4344 tm5089 |