論文 - 詳細
| RRC ID | 59392 |
|---|---|
| 著者 | Nørgaard S, Deng S, Cao W, Pocock R. |
| タイトル | Distinct CED-10/Rac1 domains confer context-specific functions in development. |
| ジャーナル | PLoS Genet |
| Abstract |
Rac GTPases act as master switches to coordinate multiple interweaved signaling pathways. A major function for Rac GTPases is to control neurite development by influencing downstream effector molecules and pathways. In Caenorhabditis elegans, the Rac proteins CED-10, RAC-2 and MIG-2 act in parallel to control axon outgrowth and guidance. Here, we have identified a single glycine residue in the CED-10/Rac1 Switch 1 region that confers a non-redundant function in axon outgrowth but not guidance. Mutation of this glycine to glutamic acid (G30E) reduces GTP binding and inhibits axon outgrowth but does not affect other canonical CED-10 functions. This demonstrates previously unappreciated domain-specific functions within the CED-10 protein. Further, we reveal that when CED-10 function is diminished, the adaptor protein NAB-1 (Neurabin) and its interacting partner SYD-1 (Rho-GAP-like protein) can act as inhibitors of axon outgrowth. Together, we reveal that specific domains and residues within Rac GTPases can confer context-dependent functions during animal development. |
| 巻・号 | 14(9) |
| ページ | e1007670 |
| 公開日 | 2018-9-1 |
| DOI | 10.1371/journal.pgen.1007670 |
| PII | PGENETICS-D-18-01418 |
| PMID | 30265669 |
| PMC | PMC6179291 |
| MeSH | Animals Animals, Genetically Modified Axons / physiology Caenorhabditis elegans / growth & development* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism* Female Glutamic Acid / genetics Glycine / genetics Male Microfilament Proteins / metabolism* Mutagenesis Nerve Tissue Proteins / metabolism* Neuronal Outgrowth / genetics* Protein Domains / genetics Protein Domains / physiology* rac GTP-Binding Proteins / genetics* rac GTP-Binding Proteins / metabolism |
| IF | 5.224 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm597 tm1949 tm6234 tm403 |