論文 - 詳細
| RRC ID | 59405 |
|---|---|
| 著者 | Saegusa K, Sato M, Morooka N, Hara T, Sato K. |
| タイトル | SFT-4/Surf4 control ER export of soluble cargo proteins and participate in ER exit site organization. |
| ジャーナル | J Cell Biol |
| Abstract |
Lipoproteins regulate the overall lipid homeostasis in animals. However, the molecular mechanisms underlying lipoprotein trafficking remain poorly understood. Here, we show that SFT-4, a Caenorhabditis elegans homologue of the yeast Erv29p, is essential for the endoplasmic reticulum (ER) export of the yolk protein VIT-2, which is synthesized as a lipoprotein complex. SFT-4 loss strongly inhibits the ER exit of yolk proteins and certain soluble cargo proteins in intestinal cells. SFT-4 predominantly localizes at ER exit sites (ERES) and physically interacts with VIT-2 in vivo, which suggests that SFT-4 promotes the ER export of soluble proteins as a cargo receptor. Notably, Surf4, a mammalian SFT-4 homologue, physically interacts with apolipoprotein B, a very-low-density lipoprotein core protein, and its loss causes ER accumulation of apolipoprotein B in human hepatic HepG2 cells. Interestingly, loss of SFT-4 and Surf4 reduced the number of COPII-positive ERES. Thus, SFT-4 and Surf4 regulate the export of soluble proteins, including lipoproteins, from the ER and participate in ERES organization in animals. |
| 巻・号 | 217(6) |
| ページ | 2073-2085 |
| 公開日 | 2018-6-4 |
| DOI | 10.1083/jcb.201708115 |
| PII | jcb.201708115 |
| PMID | 29643117 |
| PMC | PMC5987718 |
| MeSH | Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / metabolism* Egg Proteins / metabolism Endoplasmic Reticulum / metabolism* Endoplasmic Reticulum / ultrastructure Hep G2 Cells Humans Intestines / ultrastructure Membrane Proteins / metabolism* Protein Binding Protein Transport RNA Interference Solubility |
| IF | 8.891 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 18 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | |