論文 - 詳細
| RRC ID | 59461 |
|---|---|
| 著者 | Cao Z, Hao Y, Fung CW, Lee YY, Wang P, Li X, Xie K, Lam WJ, Qiu Y, Tang BZ, Shui G, Liu P, Qu J, Kang BH, Mak HY. |
| タイトル | Dietary fatty acids promote lipid droplet diversity through seipin enrichment in an ER subdomain. |
| ジャーナル | Nat Commun |
| Abstract |
Exogenous metabolites from microbial and dietary origins have profound effects on host metabolism. Here, we report that a sub-population of lipid droplets (LDs), which are conserved organelles for fat storage, is defined by metabolite-modulated targeting of the C. elegans seipin ortholog, SEIP-1. Loss of SEIP-1 function reduces the size of a subset of LDs while over-expression of SEIP-1 has the opposite effect. Ultrastructural analysis reveals SEIP-1 enrichment in an endoplasmic reticulum (ER) subdomain, which co-purifies with LDs. Analyses of C. elegans and bacterial genetic mutants indicate a requirement of polyunsaturated fatty acids (PUFAs) and microbial cyclopropane fatty acids (CFAs) for SEIP-1 enrichment, as confirmed by dietary supplementation experiments. In mammalian cells, heterologously expressed SEIP-1 engages nascent lipid droplets and promotes their subsequent expansion in a conserved manner. Our results suggest that microbial and polyunsaturated fatty acids serve unexpected roles in regulating cellular fat storage by promoting LD diversity. |
| 巻・号 | 10(1) |
| ページ | 2902 |
| 公開日 | 2019-7-1 |
| DOI | 10.1038/s41467-019-10835-4 |
| PII | 10.1038/s41467-019-10835-4 |
| PMID | 31263173 |
| PMC | PMC6602954 |
| MeSH | Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Cell Line Endoplasmic Reticulum / chemistry Endoplasmic Reticulum / genetics Endoplasmic Reticulum / metabolism* Fatty Acids / metabolism* GTP-Binding Protein gamma Subunits / genetics GTP-Binding Protein gamma Subunits / metabolism Humans Lipid Droplets / metabolism* Protein Transport |
| IF | 11.878 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 19 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4221 |