RRC ID 59498
Author Govindan JA, Jayamani E, Ruvkun G.
Title ROS-based lethality of Caenorhabditis elegans mitochondrial electron transport mutants grown on Escherichia coli siderophore iron release mutants.
Journal Proc Natl Acad Sci U S A
Abstract Caenorhabditis elegans consumes bacteria, which can supply essential vitamins and cofactors, especially for mitochondrial functions that have a bacterial ancestry. Therefore, we screened the Keio Escherichia coli knockout library for mutations that induce the C. elegans hsp-6 mitochondrial damage response gene, and identified 45 E. coli mutations that induce hsp-6::gfp We tested whether any of these E. coli mutations that stress the C. elegans mitochondrion genetically interact with C. elegans mutations in mitochondrial functions. Surprisingly, 4 E. coli mutations that disrupt the import or removal of iron from the bacterial siderophore enterobactin were lethal in combination with a collection of C. elegans mutations that disrupt particular iron-sulfur proteins of the electron transport chain. Bacterial mutations that fail to synthesize enterobactin are not synthetic lethal with these C. elegans mitochondrial mutants; it is the enterobactin-iron complex that is lethal in combination with the C. elegans mitochondrial mutations. Antioxidants suppress this inviability, suggesting that reactive oxygen species (ROS) are produced by the mutant mitochondria in combination with the bacterial enterobactin-iron complex.
Volume 116(43)
Pages 21651-21658
Published 2019-10-22
DOI 10.1073/pnas.1912628116
PII 1912628116
PMID 31591219
PMC PMC6815122
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Electron Transport / physiology* Electron Transport Chain Complex Proteins / genetics Enterobactin / biosynthesis Escherichia coli / genetics Escherichia coli / metabolism* Heat-Shock Proteins / genetics Iron / metabolism* Mitochondria / genetics Mitochondria / metabolism* Reactive Oxygen Species / metabolism Siderophores / genetics* Siderophores / metabolism
IF 9.58
Times Cited 0
C.elegans tm1133 tm1108 tm4525