論文 - 詳細
| RRC ID | 59512 |
|---|---|
| 著者 | Huggins HP, Subash JS, Stoffel H, Henderson MA, Hoffman JL, Buckner DS, Sengupta MS, Boag PR, Lee MH, Keiper BD. |
| タイトル | Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans. |
| ジャーナル | J Cell Sci |
| Abstract |
Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1, IFE-3) that bind 7-methylguanosine (m7G) mRNA caps during Caenorhabditiselegans germline development. IFE-3 plays important roles in germline sex determination (GSD), where it promotes oocyte cell fate and is dispensable for spermatogenesis. IFE-3 is expressed throughout the germline and localizes to germ granules, but is distinct from IFE-1 and PGL-1, and facilitates oocyte growth and viability. This contrasts with the robust expression in spermatocytes of IFE-1, the isoform that resides within P granules in spermatocytes and oocytes, and promotes late spermatogenesis. Each eIF4E is localized by its cognate eIF4E-binding protein (IFE-1:PGL-1 and IFE-3:IFET-1). IFE-3 and IFET-1 regulate translation of several GSD mRNAs, but not those under control of IFE-1. Distinct mutant phenotypes, in vivo localization and differential mRNA translation suggest independent dormant and active periods for each eIF4E isoform in the germline. |
| 巻・号 | 133(6) |
| 公開日 | 2020-3-30 |
| DOI | 10.1242/jcs.237990 |
| PII | jcs.237990 |
| PMID | 32079657 |
| PMC | PMC7132772 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Eukaryotic Initiation Factor-4E / genetics Male Protein Isoforms / genetics Protein Isoforms / metabolism RNA, Messenger RNA-Binding Proteins |
| IF | 4.517 |
| 引用数 | 2 |
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