RRC ID 59516
Author Kadekar P, Roy R.
Title AMPK regulates germline stem cell quiescence and integrity through an endogenous small RNA pathway.
Journal PLoS Biol
Abstract During suboptimal growth conditions, Caenorhabditis elegans larvae undergo a global developmental arrest called "dauer." During this stage, the germline stem cells (GSCs) become quiescent in an AMP-activated Protein Kinase (AMPK)-dependent manner, and in the absence of AMPK, the GSCs overproliferate and lose their reproductive capacity, leading to sterility when mutant animals resume normal growth. These defects correlate with the altered abundance and distribution of a number of chromatin modifications, all of which can be corrected by disabling components of the endogenous small RNA pathway, suggesting that AMPK regulates germ cell integrity by targeting an RNA interference (RNAi)-like pathway during dauer. The expression of AMPK in somatic cells restores all the germline defects, potentially through the transmission of small RNAs. Our findings place AMPK at a pivotal position linking energy stress detected in the soma to a consequent endogenous small RNA-mediated adaptation in germline gene expression, thereby challenging the "permeability" of the Weismann barrier.
Volume 17(6)
Pages e3000309
Published 2019-6-1
DOI 10.1371/journal.pbio.3000309
PMID 31166944
PMC PMC6576793
MeSH AMP-Activated Protein Kinases / metabolism* AMP-Activated Protein Kinases / physiology Adult Germline Stem Cells / metabolism* Adult Germline Stem Cells / physiology Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Germ Cells / metabolism Larva / genetics RNA / metabolism RNA Interference / physiology* Stem Cells / metabolism
IF 8.386
Times Cited 2
C.elegans tm1944 tm1860 tm1200