RRC ID 59518
Author Kawamura K, Maruyama IN.
Title Forward Genetic Screen for Caenorhabditis elegans Mutants with a Shortened Locomotor Healthspan.
Journal G3 (Bethesda)
Abstract Two people with the same lifespan do not necessarily have the same healthspan. One person may retain locomotor and cognitive abilities until the end of life, while another person may lose them during adulthood. Unbiased searches for genes that are required to maintain locomotor ability during adulthood may uncover key regulators of locomotor healthspan. Here, we take advantage of the relatively short lifespan of the nematode Caenorhabditis elegans and develop a novel screening procedure to collect mutants with locomotor deficits that become apparent in adulthood. After ethyl methanesulfonate mutagenesis, we isolated five C. elegans mutant strains that progressively lose adult locomotor ability. In one of the mutant strains, a nonsense mutation in elpc-2, which encodes Elongator Complex Protein Component 2, causes a progressive decline in locomotor ability during adulthood. Mutants and mutations identified in the present screen may provide insights into mechanisms of age-related locomotor impairment and the maintenance of locomotor healthspan.
Volume 9(8)
Pages 2415-2423
Published 2019-8-8
DOI 10.1534/g3.119.400241
PII g3.119.400241
PMID 31213517
PMC PMC6686916
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Genetic Association Studies Locomotion* Mutation* Phenotype*
IF 2.63
Times Cited 0
C.elegans tm2149 tm1297