Reference - Detail
|Author||Ono K, Qin Z, Johnsen RC, Baillie DL, Ono S.|
|Title||Kettin, the large actin-binding protein with multiple immunoglobulin domains, is essential for sarcomeric actin assembly and larval development in Caenorhabditis elegans.|
Among many essential genes in the nematode Caenorhabditis elegans, let-330 is located on the left arm of chromosome V and was identified as the largest target of a mutagen in this region. However, let-330 gene has not been characterized at the molecular level. Here, we report that two sequenced let-330 alleles are nonsense mutations of ketn-1, a previously characterized gene encoding kettin. Kettin is a large actin-binding protein of 472 kDa with 31 immunoglobulin domains and is expressed in muscle cells in C. elegans. let-330/ketn-1 mutants are homozygous lethal at the first larval stage with mild defects in body elongation. These mutants have severe defects in sarcomeric actin and myosin assembly in striated muscle. However, α-actinin and vinculin, which are components of the dense bodies anchoring actin to the membranes, were not significantly disorganized by let-330/ketn-1 mutation. Kettin localizes to embryonic myofibrils before α-actinin is expressed, and α-actinin deficiency does not affect kettin localization in larval muscle. Depletion of vinculin minimally affects kettin localization but significantly reduces colocalization of actin with kettin in embryonic muscle cells. These results indicate that kettin is an essential protein for sarcomeric assembly of actin filaments in muscle cells.
|MeSH||Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism Actin Cytoskeleton / ultrastructure Actinin / genetics Actinin / metabolism Actins / genetics Actins / metabolism Alleles Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Chromosomes / chemistry Codon, Nonsense Connectin / genetics* Connectin / metabolism Embryo, Nonmammalian Gene Expression Regulation, Developmental* Larva / cytology Larva / growth & development Larva / metabolism* Morphogenesis / genetics Myosins / genetics Myosins / metabolism Protein Binding Sarcomeres / genetics* Sarcomeres / metabolism Sarcomeres / ultrastructure Signal Transduction Vinculin / genetics Vinculin / metabolism Whole Genome Sequencing|