論文 - 詳細
| RRC ID | 59603 |
|---|---|
| 著者 | Sundararajan L, Smith CJ, Watson JD, Millis BA, Tyska MJ, Miller DM 3rd. |
| タイトル | Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response. |
| ジャーナル | PLoS Genet |
| Abstract |
Dendrite growth is constrained by a self-avoidance response that induces retraction but the downstream pathways that balance these opposing mechanisms are unknown. We have proposed that the diffusible cue UNC-6(Netrin) is captured by UNC-40(DCC) for a short-range interaction with UNC-5 to trigger self-avoidance in the C. elegans PVD neuron. Here we report that the actin-polymerizing proteins UNC-34(Ena/VASP), WSP-1(WASP), UNC-73(Trio), MIG-10(Lamellipodin) and the Arp2/3 complex effect dendrite retraction in the self-avoidance response mediated by UNC-6(Netrin). The paradoxical idea that actin polymerization results in shorter rather than longer dendrites is explained by our finding that NMY-1 (non-muscle myosin II) is necessary for retraction and could therefore mediate this effect in a contractile mechanism. Our results also show that dendrite length is determined by the antagonistic effects on the actin cytoskeleton of separate sets of effectors for retraction mediated by UNC-6(Netrin) versus outgrowth promoted by the DMA-1 receptor. Thus, our findings suggest that the dendrite length depends on an intrinsic mechanism that balances distinct modes of actin assembly for growth versus retraction. |
| 巻・号 | 15(6) |
| ページ | e1008228 |
| 公開日 | 2019-6-1 |
| DOI | 10.1371/journal.pgen.1008228 |
| PII | PGENETICS-D-19-00707 |
| PMID | 31220078 |
| PMC | PMC6605669 |
| MeSH | Actin Cytoskeleton / genetics Actin-Related Protein 2-3 Complex / genetics Actins / genetics* Actins / metabolism Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics* Dendritic Cells / metabolism* Membrane Proteins / genetics Myosin Heavy Chains / genetics Nerve Tissue Proteins / genetics Netrins / genetics* Neurons / metabolism* Nonmuscle Myosin Type IIB / genetics |
| IF | 5.224 |
| 引用数 | 1 |
| リソース情報 | |
| 線虫 | tm1556 |