論文 - 詳細
| RRC ID | 59625 |
|---|---|
| 著者 | Xie K, Zhang P, Na H, Liu Y, Zhang H, Liu P. |
| タイトル | MDT-28/PLIN-1 mediates lipid droplet-microtubule interaction via DLC-1 in Caenorhabditis elegans. |
| ジャーナル | Sci Rep |
| Abstract |
Ectopic lipid accumulation in lipid droplets (LD) has been linked to many metabolic diseases. In this study, DHS-3::GFP was used as a LD marker in C. elegans and a forward genetic screen was carried out to find novel LD regulators. There were 140 mutant alleles identified which were divided into four phenotypic categories: enlarged, aggregated, aggregated and small, and decreased. After genetic mapping, mutations in three known LD regulatory genes (maoc-1, dhs-28, daf-22) and a peroxisome-related gene (acox-3) were found to enlarge LDs, demonstrating the reliability of using DHS-3 as a living marker. In the screen, the cytoskeleton protein C27H5.2 was found to be involved in LD aggregation, as was the LD resident/structure-like protein, MDT-28/PLIN-1. Using yeast two-hybrid screening and pull-down assays, MDT-28/PLIN-1 was found to bind to DLC-1 (dynein light chain). Fluorescence imaging confirmed that MDT-28/PLIN-1 mediated the interaction between DHS-3 labeled LDs and DLC-1 labeled microtubules. Furthermore, MDT-28/PLIN-1 was directly bound to DLC-1 through its amino acids 1-210 and 275-415. Taken together, our results suggest that MDT-28/PLIN-1 is involved in the regulation of LD distribution through its interaction with microtubule-related proteins. |
| 巻・号 | 9(1) |
| ページ | 14902 |
| 公開日 | 2019-10-17 |
| DOI | 10.1038/s41598-019-51399-z |
| PII | 10.1038/s41598-019-51399-z |
| PMID | 31624276 |
| PMC | PMC6797801 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Dyneins / genetics Dyneins / metabolism* Gene Knockdown Techniques Lipid Droplets / metabolism* Mediator Complex / genetics Mediator Complex / metabolism* Microtubules / metabolism* Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism* Protein Binding / genetics RNA Interference |
| IF | 4.011 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1704 |