| RRC ID |
59657
|
| 著者 |
Tani H, Matsutani T, Aoki H, Nakamura K, Hamaguchi Y, Nakazato T, Hamada M.
|
| タイトル |
Identification of RNA biomarkers for chemical safety screening in neural cells derived from mouse embryonic stem cells using RNA deep sequencing analysis.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Chemical safety screening requires the development of more efficient assays that do not involve testing in animals. In vitro cell-based assays are among the most appropriate alternatives to animal testing for screening of chemical toxicity. Most studies performed to date made use of mRNAs as biomarkers. Recent studies have however indicated the presence of many unannotated non-coding RNAs (ncRNAs) in the transcriptome that do appear to encode proteins. In the present study, we performed whole-transcriptome sequencing analysis (RNA-Seq) to identify novel RNA biomarkers, including ncRNAs, which showed marked responses to the toxicity of nine chemicals. Chemical safety screening was performed in cell-based assays using mouse embryonic stem cell (mESC)-derived neural cells. Marked responses in the expression of some ncRNAs to the chemical compounds were observed. The results of the present study suggested that ncRNAs may be useful in chemical safety screening as novel RNA biomarkers.
|
| 巻・号 |
512(4)
|
| ページ |
641-646
|
| 公開日 |
2019-5-14
|
| DOI |
10.1016/j.bbrc.2018.11.141
|
| PII |
S0006-291X(18)32573-7
|
| PMID |
30497775
|
| MeSH |
Animal Testing Alternatives / methods
Animals
Cells, Cultured
Chemical Safety
Gene Expression Profiling / methods
High-Throughput Nucleotide Sequencing / methods*
Mice
Mice, Inbred C57BL
Mouse Embryonic Stem Cells / cytology
Mouse Embryonic Stem Cells / metabolism
Neurons / cytology
Neurons / drug effects*
Neurons / metabolism
Phenol / toxicity
RNA / genetics*
RNA, Untranslated / genetics
Toxicity Tests / methods*
Transcriptome / drug effects*
|
| IF |
2.705
|
| 引用数 |
1
|
| リソース情報 |
| ヒト・動物細胞 |
H-1(AES0001) |
