論文 - 詳細
| RRC ID | 59694 |
|---|---|
| 著者 | Nakanishi M, Hino M, Yoshimura M, Amakura Y, Nomoto H. |
| タイトル | Identification of sinensetin and nobiletin as major antitrypanosomal factors in a citrus cultivar. |
| ジャーナル | Exp Parasitol |
| Abstract |
Cases of human African trypanosomiasis caused by infection with a protozoan parasite, Trypanosoma brucei, are decreasing due to enhanced surveillance and control. However, effective and safe treatments for this disease are still needed. In this study, we investigated the antitrypanosomal activity of citrus fruit peel. When 19 citrus cultivars were examined for activity against T. brucei in vitro, significant activities were observed in four closely related cultivars and a distantly related one. Among these five cultivars, "Setoka" was selected for identification of its active components due to exhibiting the highest activity. Solvent extraction and gel filtration followed by preparative thin-layer chromatography succeeded in isolating two compounds exhibiting IC50s of 4.8 and 2.4 μg/mL, respectively. The spectral data of these two compounds were well consistent with those of sinensetin and nobiletin belonging to the class of polymethoxyflavones. Authentic compounds also showed similar IC50s. These results indicate that the two polymethoxyflavones are the major active components involved in the inhibition of T. brucei proliferation and are abundant in Setoka cultivar peel compared with the levels in the other cultivars. Setoka peel and the naturally occurring polymethoxyflavones might serve as dietary components imparting resistance to T. brucei. |
| 巻・号 | 200 |
| ページ | 24-29 |
| 公開日 | 2019-5-1 |
| DOI | 10.1016/j.exppara.2019.03.008 |
| PII | S0014-4894(18)30413-2 |
| PMID | 30898543 |
| MeSH | Animals Chromatography, Gel Citrus / chemistry* Dose-Response Relationship, Drug Flavones / chemistry Flavones / isolation & purification Flavones / pharmacology* Flavonoids / chemistry Flavonoids / isolation & purification Flavonoids / pharmacology* Humans Inhibitory Concentration 50 Trypanocidal Agents / chemistry Trypanocidal Agents / isolation & purification Trypanocidal Agents / pharmacology* Trypanosoma brucei brucei / drug effects* Trypanosomiasis, African / drug therapy Trypanosomiasis, African / parasitology |
| IF | 1.719 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |