RRC ID 59700
Author Sugahara M, Nakaoki Y, Yamaguchi A, Hashimoto K, Miyamoto Y.
Title Vitronectin is Involved in the Morphological Transition of Neurites in Retinoic Acid-Induced Neurogenesis of Neuroblastoma Cell Line Neuro2a.
Journal Neurochem Res
Abstract Vitronectin (Vtn), one of the extracellular matrix proteins, has been reported to result in cell cycle exit, neurite formation, and polarization of neural progenitor cells during neurogenesis. The underlying mechanism, however, has not been fully understood. In this study, we investigated the roles of Vtn and its integrin receptors, during the transition of neurites from multipolar to bipolar morphology, accompanying the cell cycle exit in neural progenitor cells. We used mouse neuroblastoma cell line Neuro2a as a model of neural progenitor cells which can induce cell cycle exit and the morphological transition of neurites by retinoic acid (RA)-stimulation. Treatment with an antibody for Vtn suppressed the RA-induced cell cycle exit and multipolar-to-bipolar transition. Furthermore, immunostaining results showed that in the cells displaying multipolar morphology Vtn was partially localized at the tips of neurites and in cells displaying bipolar morphology at both tips. This Vtn localization and multipolar-to-bipolar transition was perturbed by the transfection of a dominant negative mutant of cell polarity regulator Par6. In addition, a knockdown of β5 integrin, which is a receptor candidate for Vtn, affected the multipolar-to-bipolar transition. Taken together, these results suggest that Vtn regulates the multipolar-to-bipolar morphological transition via αvβ5 integrin.
Volume 44(7)
Pages 1621-1635
Published 2019-7-1
DOI 10.1007/s11064-019-02787-4
PII 10.1007/s11064-019-02787-4
PMID 30937689
MeSH Adaptor Proteins, Signal Transducing / metabolism Animals Antibodies / immunology Cell Cycle / drug effects Cell Line, Tumor Gene Knockdown Techniques Integrin alphaVbeta3 / metabolism Integrin beta Chains / genetics Integrin beta3 / genetics Mice Neurites / physiology* Neurogenesis / drug effects Neurogenesis / physiology* Receptors, Vitronectin / metabolism Tretinoin / pharmacology Up-Regulation Vitronectin / antagonists & inhibitors Vitronectin / genetics Vitronectin / immunology Vitronectin / metabolism*
IF 2.782
Times Cited 2
Human and Animal Cells NB2a(RCB2639)