論文 - 詳細
| RRC ID | 59701 |
|---|---|
| 著者 | Hibino M, Yamada Y, Fujishita N, Sato Y, Maeki M, Tokeshi M, Harashima H. |
| タイトル | The Use of a Microfluidic Device to Encapsulate a Poorly Water-Soluble Drug CoQ10 in Lipid Nanoparticles and an Attempt to Regulate Intracellular Trafficking to Reach Mitochondria. |
| ジャーナル | J Pharm Sci |
| Abstract |
A number of drugs that are currently on the market, as well as new candidates for drugs, are poorly water soluble. Because of this, a need exists to develop drug formulations that will permit the expanded use of such drugs. The use of liposomes and lipid nanoparticles for drug delivery has attracted attention as a technique for solubilizing molecules that are poorly water soluble, but this technique faces serious scale-up risks. In this study, we report on attempts to encapsulate Coenzyme Q10 (CoQ10) as a model of a poorly water-soluble drug in an MITO-Porter, a liposome for mitochondrial delivery using a microfluidic device (a CoQ10-MITO-Porter [μ]). The physical properties of the CoQ10-MITO-Porter [μ] including homogeneity, size, and preparation volume were compared with those for a CoQ10-MITO-Porter prepared by the ethanol dilution method (a CoQ10-MITO-Porter [ED]). In the case where a microfluidic device was used, a small-sized CoQ10-MITO-Porter was formed homogeneously, and it was possible to prepare it on a large scale. Intracellular observations using HeLa cells showed that the CoQ10-MITO-Porter [μ] was efficiently internalized by cells to reach mitochondria. These results indicate that the CoQ10-MITO-Porter [μ] represents a potential candidate for use in mitochondrial nanomedicine. |
| 巻・号 | 108(8) |
| ページ | 2668-2676 |
| 公開日 | 2019-8-1 |
| DOI | 10.1016/j.xphs.2019.04.001 |
| PII | S0022-3549(19)30214-X |
| PMID | 30959057 |
| MeSH | HeLa Cells Humans Lab-On-A-Chip Devices Lipid Metabolism Lipids / chemistry Liposomes / chemistry Liposomes / metabolism* Mitochondria / metabolism* Solubility Ubiquinone / administration & dosage Ubiquinone / analogs & derivatives* Ubiquinone / chemistry Ubiquinone / pharmacokinetics Vitamins / administration & dosage* Vitamins / chemistry Vitamins / pharmacokinetics Water / chemistry |
| IF | 3.197 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |