RRC ID 59720
Author Matsuzaki T, Nakamura M, Nogita T, Sato A.
Title Cellular Uptake and Release of Intact Lactoferrin and Its Derivatives in an Intestinal Enterocyte Model of Caco-2 Cells.
Journal Biol Pharm Bull
Abstract An Intact form of lactoferrin (LF) is known to be absorbed from the small intestine and transported into the blood circulation. We reevaluated the cellular uptake and release of LF using an enterocyte model of human small intestinal cells derived from the Caco-2 cell line. In contrast to a previous report, we observed that intact bovine LF was taken up into seven and 21 d-cultured Caco-2 cells and successfully released back into the culture medium, even though the human intestinal LF receptor, intelectin-1, was not immunochemically detectable. Similar observations were made for human LF and its derivatives (the N-terminal half of LF designated N-lobe and Fc fusions). These observations regarding the uptake and release of intact LF in Caco-2 cells were consistent with in vivo observations. Therefore, we propose that the uptake and release of intact LF by Caco-2 cells should be assessed as a potential in vitro model of in vivo LF absorption in human intestines.
Volume 42(6)
Pages 989-995
Published 2019
DOI 10.1248/bpb.b19-00011
PMID 31155596
MeSH Animals CHO Cells Caco-2 Cells Cricetulus Enterocytes / drug effects* Enterocytes / metabolism Humans Intestines / cytology* Lactoferrin / pharmacology* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Recombinant Proteins / metabolism
IF 1.54
Times Cited 2
Human and Animal Cells CACO-2(RCB0988)