RRC ID 59746
Author Chen LH, Sung TC, Lee HH, Higuchi A, Su HC, Lin KJ, Huang YR, Ling QD, Kumar SS, Alarfaj AA, Munusamy MA, Nasu M, Chen DC, Hsu ST, Chang Y, Lee KF, Wang HC, Umezawa A.
Title Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels.
Journal Biomater Sci
Abstract Recombinant vitronectin-grafted hydrogels were developed by adjusting surface charge of the hydrogels with grafting of poly-l-lysine for optimal culture of human embryonic stem cells (hESCs) under xeno- and feeder-free culture conditions, with elasticity regulated by crosslinking time (10-30 kPa), in contrast to conventional recombinant vitronectin coating dishes, which have a fixed stiff surface (3 GPa). hESCs proliferated on the hydrogels for over 10 passages and differentiated into the cells derived from three germ layers indicating the maintenance of pluripotency. hESCs on the hydrogels differentiated into cardiomyocytes under xeno-free culture conditions with much higher efficiency (80% of cTnT+ cells) than those on conventional recombinant vitronectin or Matrigel-coating dishes just only after 12 days of induction. It is important to have an optimal design of cell culture biomaterials where biological cues (recombinant vitronectin) and physical cues (optimal elasticity) are combined for high differentiation of hESCs into specific cell lineages, such as cardiomyocytes, under xeno-free and feeder-free culture conditions.
Volume 7(10)
Pages 4345-4362
Published 2019-9-24
DOI 10.1039/c9bm00418a
PMID 31411209
MeSH Cell Differentiation / physiology Cell Line Cell Proliferation / physiology Human Embryonic Stem Cells / cytology* Human Embryonic Stem Cells / metabolism Humans Hydrogels / chemistry* Myocytes, Cardiac / cytology Myocytes, Cardiac / metabolism Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism Vitronectin / chemistry*
IF 5.251
Times Cited 2
Human and Animal Cells 454E2(HPS0077)