RRC ID 59813
Author Tanaka Y, Jahan MGS, Kondo T, Nakano M, Yumura S.
Title Cytokinesis D is Mediated by Cortical Flow of Dividing Cells Instead of Chemotaxis.
Journal Cells
Abstract Cytokinesis D is known as the midwife mechanism in which neighboring cells facilitate cell division by crossing the cleavage furrow of dividing cells. Cytokinesis D is thought to be mediated by chemotaxis, where midwife cells migrate toward dividing cells by sensing an unknown chemoattractant secreted from the cleavage furrow. In this study, to validate this chemotaxis model, we aspirated the fluid from the vicinity of the cleavage furrow of a dividing Dictyostelium cell and discharged it onto a neighboring cell using a microcapillary. However, the neighboring cells did not show any chemotaxis toward the fluid. In addition, the cells did not manifest an increase in the levels of intracellular Ca2+, cAMP, or cGMP, which are expected to rise in chemotaxing cells. From several lines of our experiments, including these findings, we concluded that chemotaxis does not contribute to cytokinesis D. As an alternative, we propose a cortical-flow model, where a migrating cell attaches to a dividing cell by chance and is guided toward the furrow by the cortical flow on the dividing cell, and then physically assists the separation of the daughter cells.
Volume 8(5)
Published 2019-5-17
DOI 10.3390/cells8050473
PII cells8050473
PMID 31108912
PMC PMC6562445
MeSH Actin Cytoskeleton / metabolism Actins / metabolism Calcium / metabolism Cell Movement Cell Tracking / methods Cells, Cultured Chemotactic Factors / metabolism Chemotaxis / physiology* Cyclic AMP / metabolism Cyclic GMP / metabolism Cytokinesis / physiology* Cytoplasm / metabolism Dictyostelium / cytology* Dictyostelium / physiology* Green Fluorescent Proteins / metabolism Interphase / physiology Microscopy, Phase-Contrast Mitosis / physiology Models, Biological Pleckstrin Homology Domains / physiology
IF 5.656
Times Cited 2
Resource
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