Reference - Detail
|Author||Akashi M, Harada S, Moki S, Okouji Y, Takahashi K, Kada S, Yamagami K, Sekine Y, Watanabe S, Chibazakura T, Yoshikawa H.|
|Title||Transposition of insertion sequence IS256Bsu1 in Bacillus subtilis 168 is strictly dependent on recA.|
|Journal||Genes Genet Syst|
We developed an insertion sequence transposition detection system called the "jumping cat assay" and applied it to the Bacillus subtilis chromosome using IS256Bsu1 derived from B. subtilis natto. The high frequency of transposition enabled us to explore host factors; combining the assay and genetic analyses revealed that recA is essential for the transposition of IS256Bsu1. Detailed analyses using various domain mutants of recA demonstrated that this essentiality is not related to the function of recA in homologous recombination. Instead, the ATP binding and hydrolysis function seemed to be crucial for IS transposition. To elucidate the role of recA, we focused on the muB gene of the enterobacteriophage Mu. Based on information from the NCBI Conserved Domain Database, both MuB and RecA belong to the P-loop dNTPase superfamily. Further experiments revealed that muB complements the transposition-defective phenotype of a recA deletant, although it could not rescue UV sensitivity. These results suggest that recA shares a common function with muB that helps the transposition of IS256Bsu1 in B. subtilis.
|MeSH||Adenosine Triphosphate / metabolism Bacillus subtilis / genetics* Bacterial Proteins / genetics Bacterial Proteins / metabolism* DNA Transposable Elements* DNA-Binding Proteins / genetics Homologous Recombination Mutation Protein Binding Rec A Recombinases / genetics Rec A Recombinases / metabolism* Viral Proteins / genetics|
|Prokaryotes B. subtilis||MBS729 MBS730 MBS731 MBS732 MBS733 MBS734 MBS735 MBS736 MBS737 MBS738 MBS739 MBS740 MBS741 MBS742 MBS743 MBS744 MBS745 MBS746 MBS747|