論文 - 詳細
| RRC ID | 59849 |
|---|---|
| 著者 | Yoneda K, Kuwata T, Chikaishi Y, Mori M, Kanayama M, Takenaka M, Oka S, Hirai A, Imanishi N, Kuroda K, Ichiki Y, Ohnaga T, Tanaka F. |
| タイトル | Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma. |
| ジャーナル | Cancer Sci |
| Abstract |
Detection of rare tumor cells circulating in the blood (CTCs) presents technical challenges. CellSearch, the only approved system for clinical use, fails to capture epithelial cell adhesion molecule-negative CTCs such as malignant pleural mesothelioma (MPM). We have developed a novel microfluidic device (CTC-chip) in which any Ab to capture CTCs is conjugated. The CTC-chip was coated with an Ab against podoplanin that is abundantly expressed on MPM. Circulating tumor cell-detection performance was evaluated in experimental models in which MPM cells were spiked in blood sampled from a healthy volunteer and in clinical samples drawn from MPM patients. The CTC-chip showed superior CTC-detection performance over CellSearch in experimental models (sensitivity, 63.3%-64.5% vs 0%-1.1%; P < .001) and in clinical samples (CTC-positivity, 68.8% vs 6.3%; P < .001). A receiver operating characteristic (ROC) analysis showed that the CTC test provided a significant diagnostic performance in discrimination of unresectable disease from resectable disease (area under the ROC curve, 0.851; P = .003). The higher CTC count (≥2 cells/mL) was significantly associated with a poor prognosis (P = .030). The novel CTC-chip enabled sensitive detection of CTCs, which provided significant diagnostic and prognostic information in MPM. |
| 巻・号 | 110(2) |
| ページ | 726-733 |
| 公開日 | 2019-2-1 |
| DOI | 10.1111/cas.13895 |
| PMID | 30499156 |
| PMC | PMC6361567 |
| MeSH | Aged Aged, 80 and over Cell Count / methods Cell Line, Tumor Epithelial Cell Adhesion Molecule / metabolism Female Humans Lab-On-A-Chip Devices Lung Neoplasms / blood* Lung Neoplasms / metabolism Lung Neoplasms / pathology* Male Mesothelioma / blood* Mesothelioma / metabolism Mesothelioma / pathology* Mesothelioma, Malignant Microfluidics / methods Middle Aged Neoplastic Cells, Circulating / metabolism Neoplastic Cells, Circulating / pathology* Pleural Neoplasms / blood* Pleural Neoplasms / metabolism Pleural Neoplasms / pathology* Prognosis ROC Curve |
| IF | 4.751 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-4(RCB2293) |