RRC ID 59861
Author Haniuda K, Nojima T, Kitamura D.
Title In Vitro-Induced Germinal Center B Cell Culture System.
Journal Methods Mol Biol
Abstract In germinal centers (GCs), B cells undergo repeated cycles of proliferation and affinity-based selection, and differentiate into memory B cells or long-lived plasma cells. It has been difficult to elucidate regulatory mechanisms for the dynamic GC B cell maturation and differentiation, partly because experimental manipulation of GC B cells has been limited. Here we describe a culture system in which we can induce massive expansion of naive B cells that exhibit GC B cell-like phenotype and acquire abilities to differentiate into memory B cells or bone marrow plasma cells depending on cytokine conditions. This system will allow us to elucidate the molecular mechanisms of GC B cell differentiation.
Volume 1623
Pages 125-133
Published 2017
DOI 10.1007/978-1-4939-7095-7_11
PMID 28589353
MeSH Animals B-Lymphocytes / cytology* B-Lymphocytes / immunology B-Lymphocytes / metabolism Biomarkers Cell Culture Techniques Cell Line Cells, Cultured Cytokines / metabolism Germinal Center / cytology* Germinal Center / immunology Immunologic Memory Mice Plasma Cells / cytology Plasma Cells / immunology Plasma Cells / metabolism Spleen / cytology Spleen / immunology Spleen / metabolism
Human and Animal Cells BALB/3T3 clone A31(RCB0005)