RRC ID 59866
著者 Matsuda R, Uchitomi R, Oyabu M, Hatazawa Y, Kamei Y.
タイトル Metabolomic analysis of C2C12 myoblasts induced by the transcription factor FOXO1.
ジャーナル FEBS Lett
Abstract The transcription factor FOXO1 is considered to play roles in the regulation of energy metabolism in various tissues. To determine the metabolic changes occurring due to FOXO1 activation, we analyzed the metabolic profile of C2C12 myoblasts expressing a FOXO1-estrogen receptor fusion protein using capillary electrophoresis with electrospray ionization time-of-flight mass spectrometry (CE-TOFMS). In FOXO1-activated cells, the metabolite levels during glycolysis are higher and the gene expression of pyruvate dehydrogenase kinase, an enzyme that inhibits glucose utilization, is increased. In addition, the metabolite levels of numerous amino acids are decreased, with increased gene expression of branched chain amino acid metabolism enzymes. Our results suggest that FOXO1 suppresses glucose utilization and promotes the use of proteins/amino acids as energy sources in muscle cells, potentially during starvation.
巻・号 593(12)
ページ 1303-1312
公開日 2019-6-1
DOI 10.1002/1873-3468.13445
PMID 31111473
MeSH Animals Cell Line Electrophoresis, Capillary Forkhead Box Protein O1 / metabolism* Glucose / metabolism Mass Spectrometry Metabolomics* Mice Muscle, Skeletal / cytology Muscle, Skeletal / metabolism* Myoblasts / metabolism*
IF 2.675
引用数 2
リソース情報
ヒト・動物細胞 C2C12(RCB0987)