RRC ID 59889
Author Zhang X, Yin Y, Yue L, Gong L.
Title Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.
Journal Invest. Ophthalmol. Vis. Sci.
Abstract Purpose:Our study aimed to evaluate the side effects of selective serotonin reuptake inhibitors (SSRIs) on the ocular surface.
Methods:Twenty patients with depression and dry eye disease (DED) were randomly picked to receive SSRI treatment, whereas another 20 patients received placebo treatment. The serotonin, inflammatory cytokine, and proapoptotic protein levels were determined by using protein chip, qRT-PCR, and ELISA analyses. A rat depression model was established, and SSRIs were applied for 3 or 6 weeks. Tear production and corneal epithelial barrier function were evaluated. The serotonin and inflammatory cytokine levels were analyzed by qRT-PCR, immunohistochemical staining, and ELISA. Human corneal epithelial cells were subjected to serotonin, a HTR antagonist, and/or an NF-κB signaling inhibitor. The inflammatory cytokine and proapoptotic protein levels were determined by qRT-PCR, Western blot analysis, and ELISA. The cell apoptosis rate was assessed by using flow cytometry.
Results:The SSRI group had higher tear serotonin levels and more serious inflammation and cell apoptosis on the ocular surface. In the rat depression model, depression decreased tear secretion and increased IL-1β and TNF-α production, whereas the serotonin, TLR2, and TLR4 levels were not increased. SSRI aggravated DED, disrupted the corneal epithelial barrier, and promoted an inflammatory response on the ocular surface by increasing the tear serotonin levels. In addition, serotonin induced an inflammatory response and cell apoptosis in corneal epithelial cells by activating NF-κB signaling.
Conclusions:SSRIs aggravate depression-associated DED via activating the NF-κB pathway. The antagonist of HTRs or the inhibitor of NF-κB signaling presents a potential therapeutic strategy for depression-associated DED. (Trial registration number, ChiCTR1800015592).
Volume 60(1)
Pages 407-419
Published 2019-1-2
DOI 10.1167/iovs.18-25572
PII 2723288
PMID 30695093
MeSH Animals Blotting, Western Cells, Cultured Cytokines / metabolism Depression / chemically induced* Depression / metabolism Depression / physiopathology Dry Eye Syndromes / chemically induced* Dry Eye Syndromes / metabolism Dry Eye Syndromes / physiopathology Enzyme-Linked Immunosorbent Assay Epithelium, Corneal / physiology Flow Cytometry Humans Immunohistochemistry Male NF-kappa B / metabolism* Paroxetine / adverse effects* Rats Rats, Sprague-Dawley Real-Time Polymerase Chain Reaction Serotonin / metabolism Serotonin Uptake Inhibitors / adverse effects* Tears / metabolism
IF 3.812
Human and Animal Cells HCE-T(RCB2280)