論文 - 詳細
| RRC ID | 59909 |
|---|---|
| 著者 | Iijima M, Okonogi N, Nakajima NI, Morokoshi Y, Kanda H, Yamada T, Kobayashi Y, Banno K, Wakatsuki M, Yamada S, Kamada T, Aoki D, Hasegawa S. |
| タイトル | Significance of PD-L1 expression in carbon-ion radiotherapy for uterine cervical adeno/adenosquamous carcinoma. |
| ジャーナル | J Gynecol Oncol |
| Abstract |
OBJECTIVE:Programmed cell death-ligand 1 (PD-L1) is expressed in tumor cells and has been shown to predict clinical outcomes of several types of malignancies. The aim of this study was to investigate the effects of carbon-ion (C-ion) beam irradiation on PD-L1 expression in human uterine cervical adeno/adenosquamous carcinoma (UCAA) cells and clinical samples and to identify the prognostic factors for outcomes after C-ion radiotherapy (CIRT). METHODS:The effects of C-ion irradiation on PD-L1 expression in human UCAA and cervical squamous cell carcinoma cells were examined by flow cytometry. We examined PD-L1 expression in UCAA biopsy specimens from 33 patients before CIRT started (pre-CIRT) and after 12 Gy (relative biological effectiveness [RBE]) irradiation (post-12Gy-C) in 4 fractions of CIRT to investigate the correlation between PD-L1 status and clinical outcomes. RESULTS:The PD-L1 expression was upregulated by C-ion beam in a dose-dependent manner in HeLa and SiHa cells through phosphorylated Chk1. The overall frequencies of pre-CIRT and post-12Gy-C PD-L1 positivity were 45% (15/33) and 67% (22/33), respectively. The post-12Gy-C PD-L1 expression was significantly elevated compared to the pre-CIRT PD-L1 expression. There was no significant relationship between the pre-CIRT PD-L1 status and clinical outcomes, such as local control (LC), progression-free survival (PFS), and overall survival (OS). However, the post-12Gy-C PD-L1 expression had better correlation with PFS, but not with LC and OS. CONCLUSION:CIRT can induce PD-L1 expression in UCAA and we propose that PD-L1 expression after starting CIRT may become as a predictive prognostic marker in CIRT for UCAA. |
| 巻・号 | 31(2) |
| ページ | e19 |
| 公開日 | 2020-3-1 |
| DOI | 10.3802/jgo.2020.31.e19 |
| PII | 31.e19 |
| PMID | 31912675 |
| PMC | PMC7044016 |
| MeSH | Adult Aged B7-H1 Antigen / genetics* Biopsy Carcinoma, Adenosquamous / pathology Carcinoma, Adenosquamous / radiotherapy* Carcinoma, Squamous Cell / pathology Carcinoma, Squamous Cell / radiotherapy* Cell Line, Tumor Female Gene Expression / radiation effects* HeLa Cells Heavy Ion Radiotherapy* Humans Middle Aged Prognosis Treatment Outcome Uterine Cervical Neoplasms / pathology Uterine Cervical Neoplasms / radiotherapy* |
| IF | 3.304 |
| 引用数 | 1 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa |