論文 - 詳細
| RRC ID | 5991 |
|---|---|
| 著者 | Tamaki T, Uchiyama Y, Okada Y, Tono K, Masuda M, Nitta M, Hoshi A, Akatsuka A. |
| タイトル | Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo. |
| ジャーナル | Stem Cells Dev |
| Abstract |
The differentiation and/or therapeutic potential of skeletal muscle-derived stem cells for cardiac infarction have been studied extensively for use in cellular cardiomyoplasty, as injured cardiomyocytes exhibit limited regenerative capacity. We previously reported cardio-myogenic differentiation of skeletal muscle-derived CD34+/45(-) (Sk-34) stem cells after therapeutic transplantation. However, the clonal differentiation potential of these cells remains unknown. Here, we show that skeletal muscle-derived CD34(-)/45(-) (Sk-DN) stem cells, which are situated upstream of Sk-34 cells in the same lineage, exhibit clonal differentiation into cardiomyocytes after single cell-derived single-sphere implantation into myocardium. Sk-DN cells were enzymatically isolated from green fluorescent protein (GFP) transgenic mice and purified by flow cytometry, and were then clonally cultured in collagen-based medium with bFGF and EGF after clonal cell sorting. Single cell-derived single-sphere colonies of Sk-DN cells were directly implanted into the wild-type mouse myocardium. At 4 weeks after implantation, donor cells exhibited typical cardiomyocyte structure with the formation of gap-junctions between donor and recipient cells. Expression of specific mRNAs for cardiomyocytes, such as cardiac actin and GATA-4, Nkx2-5, Isl-1, Mef2, and Hand2, were also seen in clonal cell cultures of Sk-DN cells. Cell fusion-independent differentiation was also confirmed by bulk cell transplantation using Cre- and loxP (enhanced GFP)-mice. We conclude that Sk-DN cells can give rise to cardiac muscle cells clonally, and that skeletal muscle includes a practical cell source for cellular cardiomyoplasty. |
| 巻・号 | 19(4) |
| ページ | 503-12 |
| 公開日 | 2010-4-1 |
| DOI | 10.1089/scd.2009.0179 |
| PMID | 19634996 |
| MeSH | Actins / genetics Animals Antigens, CD34 Basic Helix-Loop-Helix Transcription Factors / genetics Biomarkers Cell Differentiation Cells, Cultured GATA4 Transcription Factor / genetics Gap Junctions Gene Expression Homeobox Protein Nkx-2.5 Homeodomain Proteins / genetics LIM-Homeodomain Proteins Leukocyte Common Antigens Mice Mice, Transgenic Muscle Fibers, Skeletal / cytology* Muscle Fibers, Skeletal / metabolism Myocardial Infarction / therapy Myocardium / metabolism Myocardium / ultrastructure Myocytes, Cardiac* / cytology Myocytes, Cardiac* / metabolism Myogenic Regulatory Factors / genetics Stem Cell Transplantation Stem Cells* / cytology Stem Cells* / metabolism Transcription Factors |
| IF | 3.153 |
| 引用数 | 11 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL TRANSPLANTATION HEMATOLOGY CELL & TISSUE ENGINEERING |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01828 RBRC02096 |