論文 - 詳細
| RRC ID | 59911 |
|---|---|
| 著者 | Nagase K, Hasegawa M, Ayano E, Maitani Y, Kanazawa H. |
| タイトル | Effect of Polymer Phase Transition Behavior on Temperature-Responsive Polymer-Modified Liposomes for siRNA Transfection. |
| ジャーナル | Int J Mol Sci |
| Abstract |
Small interfering RNAs (siRNAs) have been attracting significant attention owing to their gene silencing properties, which can be utilized to treat intractable diseases. In this study, two temperature-responsive liposomal siRNA carriers were prepared by modifying liposomes with different polymers-poly(N-isopropylacrylamide-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAAm-co-DMAPAAm)) and poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) P(NIPAAm-co-DMAAm). The phase transition of P(NIPAAm-co-DMAPAAm) was sharper than that of P(NIPAAm-co-DMAAm), which is attributed to the lower co-monomer content. The temperature dependent fixed aqueous layer thickness (FALT) of the prepared liposomes indicated that modifying liposomes with P(NIPAAm-co-DMAPAAm) led to a significant change in the thickness of the fixed aqueous monolayer between 37 °C and 42 °C; while P(NIPAAm-co-DMAAm) modification led to FALT changes over a broader temperature range. The temperature-responsive liposomes exhibited cellular uptake at 42 °C, but were not taken up by cells at 37 °C. This is likely because the thermoresponsive hydrophilic/hydrophobic changes at the liposome surface induced temperature-responsive cellular uptake. Additionally, siRNA transfection of cells for the prevention of luciferase and vascular endothelial growth factor (VEGF) expression was modulated by external temperature changes. P(NIPAAm-co-DMAPAAm) modified liposomes in particular exhibited effective siRNA transfection properties with low cytotoxicity compared with P(NIPAAm-co-DMAAm) modified analogues. These results indicated that the prepared temperature-responsive liposomes could be used as effective siRNA carriers whose transfection properties can be modulated by temperature. |
| 巻・号 | 20(2) |
| 公開日 | 2019-1-19 |
| DOI | 10.3390/ijms20020430 |
| PII | ijms20020430 |
| PMID | 30669495 |
| PMC | PMC6358841 |
| MeSH | Cell Line Cell Survival Gene Expression Gene Silencing Genes, Reporter Humans Liposomes* / chemistry Magnetic Resonance Spectroscopy Molecular Structure Molecular Weight Phase Transition* Polymers / chemistry* RNA Interference RNA, Small Interfering / genetics* Temperature* Thermodynamics Transfection* / methods |
| IF | 4.183 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |