| RRC ID |
59911
|
| 著者 |
Nagase K, Hasegawa M, Ayano E, Maitani Y, Kanazawa H.
|
| タイトル |
Effect of Polymer Phase Transition Behavior on Temperature-Responsive Polymer-Modified Liposomes for siRNA Transfection.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Small interfering RNAs (siRNAs) have been attracting significant attention owing to their gene silencing properties, which can be utilized to treat intractable diseases. In this study, two temperature-responsive liposomal siRNA carriers were prepared by modifying liposomes with different polymers-poly(N-isopropylacrylamide-co-N,N-dimethylaminopropyl acrylamide) (P(NIPAAm-co-DMAPAAm)) and poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) P(NIPAAm-co-DMAAm). The phase transition of P(NIPAAm-co-DMAPAAm) was sharper than that of P(NIPAAm-co-DMAAm), which is attributed to the lower co-monomer content. The temperature dependent fixed aqueous layer thickness (FALT) of the prepared liposomes indicated that modifying liposomes with P(NIPAAm-co-DMAPAAm) led to a significant change in the thickness of the fixed aqueous monolayer between 37 °C and 42 °C; while P(NIPAAm-co-DMAAm) modification led to FALT changes over a broader temperature range. The temperature-responsive liposomes exhibited cellular uptake at 42 °C, but were not taken up by cells at 37 °C. This is likely because the thermoresponsive hydrophilic/hydrophobic changes at the liposome surface induced temperature-responsive cellular uptake. Additionally, siRNA transfection of cells for the prevention of luciferase and vascular endothelial growth factor (VEGF) expression was modulated by external temperature changes. P(NIPAAm-co-DMAPAAm) modified liposomes in particular exhibited effective siRNA transfection properties with low cytotoxicity compared with P(NIPAAm-co-DMAAm) modified analogues. These results indicated that the prepared temperature-responsive liposomes could be used as effective siRNA carriers whose transfection properties can be modulated by temperature.
|
| 巻・号 |
20(2)
|
| 公開日 |
2019-1-19
|
| DOI |
10.3390/ijms20020430
|
| PII |
ijms20020430
|
| PMID |
30669495
|
| PMC |
PMC6358841
|
| MeSH |
Cell Line
Cell Survival
Gene Expression
Gene Silencing
Genes, Reporter
Humans
Liposomes* / chemistry
Magnetic Resonance Spectroscopy
Molecular Structure
Molecular Weight
Phase Transition*
Polymers / chemistry*
RNA Interference
RNA, Small Interfering / genetics*
Temperature*
Thermodynamics
Transfection* / methods
|
| IF |
4.183
|
| 引用数 |
6
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
