RRC ID 59913
著者 Takeuchi T, Tamura M, Ishiwata K, Hamasaki M, Hamano S, Arata Y, Hatanaka T.
タイトル Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactoseβ1-4fucose glyco-epitope.
ジャーナル Glycobiology
Abstract Galactoseβ1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactoseβ1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. We found that mammalian galectin-2 (Gal-2) also has a comparable glutamate residue, Glu52. In the present study, we investigated the potential nematode-suppressing activity of Gal-2 using C. elegans as a model and focusing on Gal-2 binding to the GalFuc epitope. Gal-2 suppressed C. elegans development whereas its E52D mutant (Glu52 substituted by Asp), galectin-1 and galectin-3 had little effect on C. elegans growth. Lectin-staining using fluorescently-labeled Gal-2 revealed that, like CGL2, it specifically binds to the C. elegans intestine. Natural C. elegans glycoconjugates were specifically bound by immobilized Gal-2. Western blotting with anti-GalFuc antibody showed that the bound glycoconjugates had the GalFuc epitope. Frontal affinity chromatography with pyridylamine-labeled C. elegans N-glycans disclosed that Gal-2 (but not its E52D mutant) recognizes the GalFuc epitope. Gal-2 also binds to the GalFuc-bearing glycoconjugates of Ascaris and the GalFuc epitope is present in the parasitic nematodes Nippostrongylus brasiliensis and Brugia pahangi. These results indicate that Gal-2 suppresses C. elegans development by binding to its GalFuc epitope. The findings also imply that Gal-2 may prevent infestations of various parasitic nematodes bearing the GalFuc epitope.
巻・号 29(6)
ページ 504-512
公開日 2019-6-1
DOI 10.1093/glycob/cwz022
PII 5381599
PMID 30874734
MeSH Animals Ascaris suum / growth & development Ascaris suum / metabolism Binding Sites Biomphalaria Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Disaccharides / chemistry* Disaccharides / metabolism Epitopes / chemistry* Epitopes / metabolism Galectin 2 / chemistry Galectin 2 / metabolism* HeLa Cells Humans Mice Mice, Inbred ICR
IF 4.194
引用数 2
リソース情報
ヒト・動物細胞 HeLa(RCB0007)