| RRC ID |
59914
|
| 著者 |
Khanmohammadi M, Sakai S, Taya M.
|
| タイトル |
Characterization of encapsulated cells within hyaluronic acid and alginate microcapsules produced via horseradish peroxidase-catalyzed crosslinking.
|
| ジャーナル |
J Biomater Sci Polym Ed
|
| Abstract |
Hydrogel microcapsules having the ability to promote cell adhesion and proliferation are a useful tool for fabricating tissue in vitro. The present study explored the effects of two anionic polysaccharide hydrogel membranes which have an impact on the adhesiveness, morphology and growth of cells. Microcapsules were made by coating a cell-laden gelatin microparticle with a hydrogel membrane produced from modified hyaluronic acid or alginate possessing phenolic hydroxyl moieties (HA-Ph or Alg-Ph respectively) via a horseradish peroxidase-catalyzed crosslinking reaction. Some gelatin was retained within the microcapsules to support the attachment and growth of encapsulated cells. The morphological and functional characteristics of encapsulated HeLa and 10T1/2 cells were evaluated. The HA-Ph hydrogel, which exhibited greater retention of gelatin, showed a higher degree of cytocompatibility with respect to cell adhesion, spreading and proliferation compared with the Alg-Ph hydrogel membrane. These findings indicate that HA-Ph microcapsules synthesized around a temporary gelatin microparticles are a promising cell vehicle for tissue engineering applications.
|
| 巻・号 |
30(4)
|
| ページ |
295-307
|
| 公開日 |
2019-3-1
|
| DOI |
10.1080/09205063.2018.1562637
|
| PMID |
30593259
|
| MeSH |
Alginates / chemistry*
Animals
Biocompatible Materials / chemistry*
Capsules / chemistry*
Catalysis
Cell Adhesion
Cell Line, Tumor
Cross-Linking Reagents / chemistry
Gelatin / chemistry
Horseradish Peroxidase / chemistry*
Humans
Hyaluronic Acid / chemistry*
Hydrogels / chemistry*
Mice
Tissue Engineering*
|
| IF |
2.121
|
| 引用数 |
8
|
| リソース情報 |
| ヒト・動物細胞 |
HeLa(RCB0007) |
