| RRC ID |
59924
|
| Author |
Hirama H, Satoh T, Sugiura S, Shin K, Onuki-Nagasaki R, Kanamori T, Inoue T.
|
| Title |
Glass-based organ-on-a-chip device for restricting small molecular absorption.
|
| Journal |
J Biosci Bioeng
|
| Abstract |
The use of organ-on-a-chip (OOC) devices is a promising alternative to existing cell-based assays and animal testing in drug discovery. A rapid prototyping method with polydimethylsiloxane (PDMS) is widely used for developing OOC devices. However, because PDMS tends to absorb small hydrophobic molecules, the loss of test compounds in cell-based assays and increases in background fluorescence during observation often lead to biased results in cell-based assays. To address this issue, we have fabricated a glass-based OOC device and characterized the medium flow and molecular absorption properties in comparison with PDMS-based devices. Consequently, we revealed that the glass device generated a stable medium flow, restricted the absorption of small hydrophobic molecules, and showed enhanced cell adhesiveness. This glass device is expected to be applicable to precise cell-based assays to evaluate small hydrophobic molecules, for which PDMS devices cannot be applied because of their absorption of small hydrophobic molecules.
|
| Volume |
127(5)
|
| Pages |
641-646
|
| Published |
2019-5-1
|
| DOI |
10.1016/j.jbiosc.2018.10.019
|
| PII |
S1389-1723(18)30641-8
|
| PMID |
30473393
|
| MeSH |
Adsorption
Animals
Biological Assay / instrumentation*
Cell Adhesion
Cell Line
Dimethylpolysiloxanes / chemistry
Glass / chemistry
Humans
Hydrophobic and Hydrophilic Interactions
Lab-On-A-Chip Devices*
|
| IF |
2.032
|
| Times Cited |
4
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1886) |
