| RRC ID |
59944
|
| 著者 |
Kubo S, Takagi-Kimura M, Kasahara N.
|
| タイトル |
Efficient tumor transduction and antitumor efficacy in experimental human osteosarcoma using retroviral replicating vectors.
|
| ジャーナル |
Cancer Gene Ther
|
| Abstract |
Retroviral replicating vectors (RRVs) have achieved efficient tumor transduction and enhanced therapeutic benefit in a wide variety of cancer models. Here, we evaluated two different RRVs derived from amphotropic murine leukemia virus (AMLV) and gibbon ape leukemia virus (GALV), which utilize different cellular receptors (PiT-2 and PiT-1, respectively) for viral entry, in human osteosarcoma cells. Quantitative RT-PCR showed that low levels of expression of both receptors were observed in normal and non-malignant cells. However, high PiT-2 (for AMLV) and low PiT-1 (for GALV) expression was observed in most osteosarcoma cell lines. Accordingly, AMLV expressing the green fluorescent protein gene infected and replicated more efficiently than GALV in most osteosarcoma cell lines. Furthermore, RRVs expressing the cytosine deaminase prodrug activator gene showed differential cytotoxicity that correlated with the results of viral spread. AMLV-RRV-mediated prodrug activator gene therapy achieved significant inhibition of subcutaneous MG-63 tumor growth over GALV in nude mice. These data indicate that AMLV vectors predominate over GALV in human osteosarcoma cells. Moreover, our findings support the potential utility of the two RRVs in personalized cancer virotherapy on the basis of receptor expression.
|
| 巻・号 |
26(1-2)
|
| ページ |
41-47
|
| 公開日 |
2019-2-1
|
| DOI |
10.1038/s41417-018-0037-y
|
| PII |
10.1038/s41417-018-0037-y
|
| PMID |
30042500
|
| PMC |
PMC6760559
|
| MeSH |
Animals
Bone Neoplasms / metabolism
Bone Neoplasms / therapy*
Cell Line, Tumor
Cytosine Deaminase
Female
Humans
Leukemia Virus, Gibbon Ape*
Leukemia Virus, Murine*
Mice
Mice, Nude
Oncolytic Virotherapy*
Osteosarcoma / metabolism
Osteosarcoma / therapy*
Prodrugs
Receptors, Virus*
Treatment Outcome
Xenograft Model Antitumor Assays
|
| IF |
4.681
|
| 引用数 |
4
|
| リソース情報 |
| ヒト・動物細胞 |
HOS(RCB0992)
MG-63(RCB1890)
Saos-2(RCB0428) |
