RRC ID 59980
Author Diaz Verdugo C, Myren-Svelstad S, Aydin E, Van Hoeymissen E, Deneubourg C, Vanderhaeghe S, Vancraeynest J, Pelgrims R, Cosacak MI, Muto A, Kizil C, Kawakami K, Jurisch-Yaksi N, Yaksi E.
Title Glia-neuron interactions underlie state transitions to generalized seizures.
Journal Nat Commun
Abstract Brain activity and connectivity alter drastically during epileptic seizures. The brain networks shift from a balanced resting state to a hyperactive and hypersynchronous state. It is, however, less clear which mechanisms underlie the state transitions. By studying neural and glial activity in zebrafish models of epileptic seizures, we observe striking differences between these networks. During the preictal period, neurons display a small increase in synchronous activity only locally, while the gap-junction-coupled glial network was highly active and strongly synchronized across large distances. The transition from a preictal state to a generalized seizure leads to an abrupt increase in neural activity and connectivity, which is accompanied by a strong alteration in glia-neuron interactions and a massive increase in extracellular glutamate. Optogenetic activation of glia excites nearby neurons through the action of glutamate and gap junctions, emphasizing a potential role for glia-glia and glia-neuron connections in the generation of epileptic seizures.
Volume 10(1)
Pages 3830
Published 2019-8-23
DOI 10.1038/s41467-019-11739-z
PII 10.1038/s41467-019-11739-z
PMID 31444362
PMC PMC6707163
MeSH Animals Animals, Genetically Modified Brain / cytology Brain / diagnostic imaging Brain / physiopathology* Cell Communication* Cortical Excitability / physiology* Disease Models, Animal Epilepsy / physiopathology* Gap Junctions / physiology Glutamic Acid / metabolism Humans Microscopy, Confocal Nerve Net / cytology Nerve Net / physiopathology Neuroglia / physiology Neurons / physiology Optical Imaging Optogenetics Patch-Clamp Techniques Seizures / physiopathology* Zebrafish
IF 11.878
Times Cited 13
Resource
Zebrafish Tg(gad1b:GFP) Tg(vglut2a:lRl-GFP)