| RRC ID |
59990
|
| Author |
Paerhati P, Ito A, Yoshioka K, Iwamoto K, Fujiwara S, Horie M, Kawabe Y, Kamihira M.
|
| Title |
Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells.
|
| Journal |
J Biosci Bioeng
|
| Abstract |
Investigating neural differentiation of pluripotent stem cells, including induced pluripotent stem (iPS) cells, is of importance for studying early neural development and providing a potential source of cells for nerve regeneration. Stromal cell-derived inducing activity (SDIA) using PA6 stromal cells promotes neural differentiation of iPS cells. Thus, we hypothesized that cadherin gene-engineered PA6 feeder cells will enhance the performance of SDIA by facilitating cell-cell interactions. Consequently, we created cadherin gene-engineered PA6 cells. Efficiency of neural differentiation from mouse iPS cells on PA6 feeder cells overexpressing E-cadherin gene (46%) or N-cadherin gene (27%) was significantly higher compared with parental PA6 feeder cells (19%). In addition, efficiency of motor neuron differentiation from mouse iPS cells on cadherin-gene engineered feeder cells (E-cadherin, 7.4%; N-cadherin, 11%) was significantly higher compared with parental PA6 feeder cells (4.1%). Altogether, these results indicate that cadherin gene-engineered feeder cells are a potent tool for promoting neural differentiation of pluripotent stem cells.
|
| Volume |
127(5)
|
| Pages |
633-640
|
| Published |
2019-5-1
|
| DOI |
10.1016/j.jbiosc.2018.10.009
|
| PII |
S1389-1723(18)30643-1
|
| PMID |
30391238
|
| MeSH |
Animals
Cadherins / genetics
Cadherins / metabolism
Cell Communication
Cell Differentiation*
Feeder Cells / cytology*
Feeder Cells / metabolism
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Mice
Neurogenesis
Pluripotent Stem Cells / cytology
Stromal Cells / cytology*
Stromal Cells / metabolism
|
| IF |
2.032
|
| Times Cited |
0
|
| Resource |
| Human and Animal Cells |
iPS-MEF-Ng-20D-17(APS0001)
MC3T3-G2/PA6(RCB1127) |
